Pesticide-induced DNA damage is primarily repaired by base excision repair (BER) pathway. However, polymorphism in DNA repair genes may modulate individual’s DNA repair capacity (DRC) leading to increased genotoxicity and adverse health effects. Our first study in North-West Indian population aimed to evaluate the impact of OGG1 rs1052133 (Ser326Cys; C1245G), XRCC1 rs1799782 (Arg194Trp; C26304T) and XRCC1 rs25487 (Arg399Gln; G28152A) polymorphisms on the modulation of pesticide-induced DNA damage in a total of 450 subjects (225 pesticide-exposed agricultural workers and 225 age- and sex-matched controls). DNA damage was estimated by alkaline comet assay using silver-staining method. Genotyping was carried out by PCR-RFLP using site-specific restriction enzymes. Mann-Whitney U-test revealed elevation in DNA damage parameters (p < 0.01) in pesticide-exposed agricultural workers than controls. Chi-square test showed significant (p < 0.05) differences in the XRCC1 Arg194Trp (C26304T) and Arg399Gln (G28152A) genotypes among two groups. Multivariate logistic-regression analysis revealed that heterozygous genotypes of OGG1 rs1052133 (326Ser/Cys; 1245CA), XRCC1 rs1799782 (194Arg/Trp; 26304CT) and XRCC1 rs25487 (399Arg/Gln; 2815GA) were positively associated (p < 0.05) with elevated DNA damage parameters in pesticide-exposed agricultural workers. Our results strongly indicate significant positive association of variant OGG1 and XRCC1 genotypes with reduced DRC and higher pesticide-induced DNA damage in North-West Indian agricultural workers.

农药诱导的DNA损伤主要通过碱基切除修复（BER）途径修复。但是，DNA修复基因中的多态性可能会调节个体的DNA修复能力（DRC），从而导致遗传毒性增加和不利的健康影响。我们在西北印度人口中的第一项研究旨在评估OGG1 rs1052133（Ser326Cys; C1245G），XRCC1 rs1799782（Arg194Trp; C26304T）和XRCC1的影响rs25487（Arg399Gln; G28152A）多态性对总共450位受试者（225位接触农药的农业工人和225位年龄和性别相匹配的对照）的农药引起的DNA损伤的调节作用。使用银染色法通过碱彗星试验评估DNA损伤。使用位点特异性限制酶通过PCR-RFLP进行基因分型。Mann-Whitney U检验显示， 暴露于农药的农业工人的DNA损伤参数高于对照组（p <0.01）。卡方检验显示两组 的XRCC1 Arg194Trp（C26304T）和Arg399Gln（G28152A）基因型之间存在显着（p <0.05）差异。多元logistic回归分析显示OGG1的杂合基因型rs1052133（326Ser / Cys; 1245CA），XRCC1 rs1799782（194Arg / Trp; 26304CT）和XRCC1 rs25487（399Arg / Gln; 2815GA  ）与暴露于农药的农业工人的DNA损伤参数升高呈正相关（p <0.05）。我们的结果有力地表明，在印度西北部的农业工人中，OGG1和XRCC1基因型变异与DRC降低和农药引起的DNA损伤较高显着正相关。