Abstract Ozone is effective against bacteria and viruses although its influence over bioaerosols is understudied and could be useful particularly in agricultural buildings such as swine confinement buildings. Ozone treatment of air within ventilation plenum could be applied for biosecurity purposes during the quarantine of incoming animals. In this study, a bacteriophage (PhiX174) was used as a surrogate for eukaryotic viruses in nebulization experiments inside a wind tunnel to study the factors that affect ozone’s efficacy to reduce the virus. A tunnel system was installed in the workshop of a swine building with controlled relative humidity (40% and 80%), ozone concentration (0, 0.3, 0.6, 0.9, 1.2, 1.5, and 1.8 ppm), and exposure time (up to approximately 6 min) of PhiX174 nebulized. Although there was no effect of ozone on phage genomes, culturable phages were inactivated. There was a reduction of PhiX174 infectious ratios with increasing ozone concentrations and stronger effects of ozone observed at 80% relative humidity. The data suggests the potential success of a wind tunnel and ozone air treatment to control infectious viruses emitted from swine barns or quarantine buildings. Copyright © 2020 American Association for Aerosol Research

中文翻译：

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风洞中的臭氧处理以减少猪舍中的空气传播病毒

摘要 臭氧对细菌和病毒有效，尽管其对生物气溶胶的影响尚未得到充分研究，并且可能特别适用于农用建筑，例如猪圈舍。通风室内空气的臭氧处理可用于在传入动物检疫期间的生物安全目的。在这项研究中，在风洞内的雾化实验中，噬菌体（PhiX174）被用作真核病毒的替代物，以研究影响臭氧减少病毒效力的因素。在控制相对湿度（40% 和 80%）、臭氧浓度（0、0.3、0.6、0.9、1.2、1.5 和 1.8 ppm）和暴露时间（高达大约 6 分钟）的 PhiX174 雾化。虽然臭氧对噬菌体基因组没有影响，可培养的噬菌体被灭活。随着臭氧浓度的增加和在 80% 相对湿度下观察到的臭氧作用更强，PhiX174 感染率降低。数据表明，风洞和臭氧空气处理在控制猪舍或检疫建筑物散发的传染性病毒方面可能取得成功。版权所有 © 2020 美国气溶胶研究协会