The aim of this study was to evaluate the effects of different concentrations of lycopene and cysteamine on characteristics of sperm, liquid peroxidation and enzymatic activities in seminal plasma of canine semen preserved at 5°C for 72 hr. The semen samples were divided into eight aliquots: control, control sham (dimethyl sulfoxide 5%), lycopene groups (250, 500 and 750 µg/ml) and cysteamine groups (2.5, 5 and 10 mM). Motility, viability, membrane integrity, DNA integrity, total antioxidant capacity (TAC) and malondialdehyde (MDA) levels were evaluated. Progressive motility and total motility were higher with the 500 and 750 µg/ml lycopene concentrations, respectively, compared to the control group and the cysteamine groups following 72 hr of storage in the liquid storage. Motility characteristics, viability and hypo‐osmotic swelling test (HOST) percentages were significantly improved in 500 µg/ml lycopene compared to other groups. The 500 and 750 µg/ml lycopene concentrations, respectively, showed significantly reduced percentages of spermatozoa with DNA integrity compared to the control group. The 500 and 750 µg/ml lycopene concentrations, respectively, led to the significant decrease of MDA levels. The 500 µg/ml lycopene enhanced TAC levels after 48 and 72 hr that was not observed in other groups. In conclusion, the findings of this study showed that lycopene supplementation in canine semen extenders improved canine semen parameters and TAC levels and decreased MDA levels in the chilling process.

中文翻译：

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番茄红素和半胱胺补充剂对犬精液冷藏中精子和氧化应激参数的影响评估。

这项研究的目的是评估不同浓度的番茄红素和半胱胺对5℃下保存72小时的精液精液精子特性，液体过氧化和酶活性的影响。精液样品分为八个等分试样：对照，对照假（5％二甲基亚砜），番茄红素基团（250、500和750 µg / ml）和半胱胺基团（2.5、5和10 mM）。评估了运动能力，生存力，膜完整性，DNA完整性，总抗氧化剂能力（TAC）和丙二醛（MDA）水平。分别在500和750μg/ ml番茄红素浓度下，在液体储存72小时后，与对照组和半胱胺组相比，渐进运动和总运动较高。动力特性 与其他组相比，在500 µg / ml番茄红素中，活力和低渗透溶胀试验（HOST）百分比显着提高。与对照组相比，分别以500和750 µg / ml番茄红素的浓度显示具有DNA完整性的精子百分比显着降低。番茄红素的浓度分别为500和750 µg / ml，导致MDA含量显着下降。500 µg / ml番茄红素可在48和72小时后提高TAC水平，这在其他组中未观察到。总之，这项研究的结果表明，在犬精液补充剂中添加番茄红素可改善犬精液参数和TAC水平，并在冷却过程中降低MDA水平。与对照组相比，分别显示具有DNA完整性的精子百分比显着降低。番茄红素的浓度分别为500和750 µg / ml，导致MDA含量显着下降。500 µg / ml番茄红素可在48和72小时后提高TAC水平，这在其他组中未观察到。总之，这项研究的结果表明，在犬精液补充剂中添加番茄红素可改善犬精液参数和TAC水平，并在冷却过程中降低MDA水平。与对照组相比，分别显示具有DNA完整性的精子百分比显着降低。番茄红素的浓度分别为500和750 µg / ml，导致MDA含量显着下降。500 µg / ml番茄红素可在48和72小时后提高TAC水平，这在其他组中未观察到。总之，这项研究的结果表明，在犬精液补充剂中添加番茄红素可改善犬精液参数和TAC水平，并在冷却过程中降低MDA水平。