• Seed dormancy controls the timing of germination and plays a significant role in adaptation and evolution of seed plants.
• In this study, a yeast two‐hybrid, pull‐down assay and co‐immunoprecipitation assay were used to ascertain the protein relationship of SUVH5 and HDA19. Both qRT‐PCR and ChIP‐qPCR were used to examine the molecular mechanism of how HDA19 and SUVH5 regulate seed dormancy.
• The results demonstrated that histone methyltransferase SUVH5 interacted with histone deacetylase HDA19 in vivo and in vitro. In addition, they showed that mutants of HDA19 could deepen seed dormancy, and that SUVH5 had the same effect. The hda19 suvh5 double mutant displayed a higher level of seed dormancy than the single mutants hda19 or suvh5. Moreover, the expression of seed dormancy‐related genes increased in hda19, suvh5 and in hda19 suvh5 double mutant plants, which was associated with increased histone H3 acetylation (H3ac), but decreased histone H3 Lys 9 dimethylation (H3K9me2). ChIP assays proved that HDA19 could directly integrate into the chromatin of genes regulating seed dormancy.
• Taken together, our results show that HDA19 and SUVH5 work together and have a negative role in seed dormancy.

中文翻译：

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组蛋白脱乙酰基酶HDA19与组蛋白甲基转移酶SUVH5相互作用，调节拟南芥中的种子休眠。

• 种子休眠控制发芽的时间，并在种子植物的适应和进化中起重要作用。
• 在这项研究中，使用酵母双杂交，下拉测定和免疫共沉淀测定来确定SUVH5和HDA19的蛋白质关系。qRT-PCR和ChIP-qPCR均用于检查HDA19和SUVH5如何调节种子休眠的分子机制。
• 结果表明，组蛋白甲基转移酶SUVH5在体内和体外与组蛋白脱乙酰酶HDA19相互作用。此外，他们表明HDA19突变体可以加深种子休眠，而SUVH5具有相同的作用。所述hda19 suvh5双突变体显示种子休眠比单突变体的较高水平hda19或suvh5。此外，hda19，suvh5和hda19 suvh5中种子休眠相关基因的表达增加双突变体植物，与组蛋白H3乙酰化（H3ac）增加有关，但组蛋白H3 Lys 9甲基化（H3K9me2）减少。ChIP分析证明，HDA19可以直接整合到调节种子休眠基因的染色质中。
• 两者合计，我们的结果表明，HDA19和SUVH5协同工作，对种子休眠具有负面作用。