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Magnetic mesoporous carbon material based electrochemical sensor for rapid detection of penicillin sodium in milk
Journal of Food Science ( IF 3.9 ) Pub Date : 2020-07-09 , DOI: 10.1111/1750-3841.15328
Li Wang 1 , Yi Xiu 1 , Baoqing Han 1 , Lu Liu 1 , Xiaodi Niu 1 , Hongsu Wang 1
Affiliation  

In recent years, to increase growth rate and prevent infectious diseases, an excessive use of antibiotics in livestock breeding processes has resulted in the presence of antibiotic residues in animal foods. In this experiment, a new kind of electrochemical sensor is prepared based on magnetic mesoporous hollow carbon microspheres (MHM) as a penicillinase (Pen X) adsorption carrier to rapidly detect penicillin sodium (Pen G), named Pen X/MHM/MGCE. The MHM-adsorbed penicillinase can be separated from the solution by magnetic attraction and fixed on a magnetic glassy carbon electrode by physical adsorption, which is easy to operate and avoids the interference of a crosslinking agent at the active site of the enzyme. A differential pulse voltammetry (DPV) method is used to immerse the working electrode in test samples containing different concentrations of penicillin sodium solution with 0.5 mg/mL oxidized hematoxylin. According to the quantitative relationship between the current value and the concentration of penicillin sodium, the concentration of penicillin sodium in the tested samples can be determined. The detection range of the sensor is 10-8 to 10-2 mg/mL, the linear relationship is good (R2 = 0.9983), and the detection limit (LOD) is 2.655 × 10-7 mg/mL (S/N = 3). The detection of penicillin sodium in milk using a standard addition method shows good recovery. Furthermore, the proposed method has the advantages of a wide detection range, good enzyme immobilization capacity, good precision and stability, convenient cleaning, and recycling of solid materials. Thus, it can successfully achieve rapid detection in milk samples. PRACTICAL APPLICATION: The sensor provides a low detection limit and high recovery rate of electrode material; therefore, the sensor can realize the rapid and quantitative detection of penicillin sodium in milk.

中文翻译:

基于磁性介孔碳材料的电化学传感器快速检测牛奶中青霉素钠

近年来,为了提高生长速度和预防传染病,牲畜养殖过程中过度使用抗生素导致动物食品中存在抗生素残留。本实验以磁性介孔空心碳微球(MHM)为青霉素酶(Pen X)吸附载体,制备了一种新型电化学传感器,用于快速检测青霉素钠(Pen G),命名为Pen X/MHM/MGCE。吸附了MHM的青霉素酶可以通过磁引力从溶液中分离出来,通过物理吸附固定在磁性玻碳电极上,操作方便,避免了酶活性位点交联剂的干扰。使用差分脉冲伏安法 (DPV) 方法将工作电极浸入含有不同浓度青霉素钠溶液和 0​​.5 mg/mL 氧化苏木素的测试样品中。根据电流值与青霉素钠浓度的定量关系,可确定被测样品中青霉素钠的浓度。该传感器的检测范围为10-8~10-2 mg/mL,线性关系良好(R2 = 0.9983),检测限(LOD)为2.655×10-7 mg/mL(S/N = 3)。使用标准添加方法检测牛奶中的青霉素钠显示出良好的回收率。此外,该方法具有检测范围广、酶固定化能力好、精密度和稳定性好、清洗方便、和回收固体材料。因此,它可以成功地实现对牛奶样品的快速检测。实际应用:该传感器提供低检测限和高电极材料回收率;因此,该传感器可实现牛奶中青霉素钠的快速定量检测。
更新日期:2020-07-09
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