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Comparative foliar anatomical and pollen morphological studies of Acanthaceae using light microscope and scanning electron microscope for effective microteaching in community.
Microscopy Research and Technique ( IF 2.5 ) Pub Date : 2020-07-08 , DOI: 10.1002/jemt.23502
Jamil Raza 1 , Mushtaq Ahmad 1 , Muhammad Zafar 1 , Mohammad Athar 2 , Shazia Sultana 1 , Salman Majeed 1 , Ghulam Yaseen 1, 3 , Muhammad Imran 4 , Moona Nazish 1 , Amir Hussain 1
Affiliation  

In this study, foliar anatomy and pollen morphology of 10 species of Acanthaceae has been investigated using light and scanning electron microscopy. The study was aimed to highlight the role of microscopy in microteaching at community for proper characterization of plants using palyno‐anatomical characters including pollen type, exine sculpturing, shape of epidermal cells, pattern of anticlinal wall, type and size of stomata, and trichome. Most of the species have polygonal cell shapes but some species have irregular, tetragonal, and pentagonal shape of epidermal cells. The largest epidermal cell length on adaxial and abaxial surface were observed in Asystasia gangetica 66.95 and 87.40 μm whereas least was observed on adaxial surface in Justicia adhatoda 36.9 μm and on abaxial surface in Barleria cristata 35.65 μm. In anatomy, species have diacytic type of stomata, whereas stomata of paracytic type observed in two species, while in A. gangetica cyclocytic type of stomata are present. Quantitively on abaxial surface, largest stomata length 29.9 μm and width 24.30 μm was noted in B. cristata. While shortest stomata length was observed in Ruellia prostrata 25.95 μm whereas minimum width of stomata was examined in Barleria acanthoides 2.05 μm. The diversity of trichomes are present in all species except in Ruellia brittoniana. Acanthaceae can be characterized by exhibiting different pollen morphology having five types of pollen shapes, prolate, spheroidal, perprolate, subprolate, and oblate spheroidal. Exine peculiarities showing variations such as reticulate, granulate, coarsely reticulate, lophoreticulate, perforate tectate, and granulate surface were examined.

中文翻译:

利用光学显微镜和扫描电子显微镜对老鼠科的叶解剖和花粉形态进行比较研究,以有效地进行社区微教学。

在这项研究中,使用光学和扫描电子显微镜研究了10种棘皮科的叶解剖和花粉形态。这项研究旨在强调显微镜在社区微教学中的作用,该系统利用花粉解剖特征(包括花粉类型,外壁雕刻,表皮细胞形状,抗斜壁模式,气孔的类型和大小以及毛状体)正确表征植物。大多数种类具有多边形细胞形状,但有些种类具有不规则,四边形和五边形的表皮细胞形状。在未观察到对正面和背面的最大表皮细胞长度宽叶十万错66.95和87.40微米,而至少是正面表面上观察到在鸭嘴花36.9μm,在Barleria cristata的背面35.65μm。在解剖,物种具有横列型气孔,而平列型的气孔在两个物种观察到,而在A.河豚环列型气孔的存在。在背面,定量地观察到在B. cristata中最大的气孔长度为29.9μm,宽度为24.30μm。虽然在Ruellia prostrata中观察到最短的气孔长度为25.95μm,而在Barleria acanthoides 2.05μm中观察到最小的气孔宽度。毛状体的多样性在所有物种中都存在,除了Brutlia brittoniana。棘皮科的特征在于表现出具有五种花粉形状的不同花粉形态,这些花粉形状为扁长形,球形,过扁形,近扁形和扁球形。检查显示出网状,颗粒状,粗网状,菱形,穿孔状网状和颗粒表面变化的外来异物。
更新日期:2020-07-08
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