As a traditional Chinese medicine, Marsdenia tenacissima (Roxb.) Wight et Arn. plays an indispensable role in clinical practice owing to its specific efficacy in treating malignant tumors, leukocythemia, cystitis and asthma. This study aimed to establish a novel and scientific LC–MS/MS approach to simultaneously determine tenacissoside B, H, G and I, caffeic acid, cryptochlorogenic acid, chlorogenic acid and neochlorogenic acid from M. tenacissima extract within the rat plasma samples. Digoxin was used as the internal reference. All determinations were carried out using the Eclipse Plus C₁₈ column, and water (containing 0.1% formic acid) was used as the mobile phase A, while acetonitrile was the mobile phase B for gradient elution. The UPLC methods were validated, including calibration curves, accuracy, precision, stability and recovery of the total eight analytes, in accordance with the requirements for biopharmaceutical analysis. Moreover, the proposed approach was also used in comprehensive pharmacokinetic research on those eight analytes in rats following M. tenacissima extract gavage. According to the pharmacokinetic parameters, tenacissoside B, I, H and G are the long‐acting and primary bioactive constituents in M. tenacissima extract, with long mean residence times and high concentrations. Our findings shed light on the absorption mechanism and provide significant information for the clinical application of M. tenacissima.

中文翻译：

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用LC-MS / MS同时测定大鼠血浆中的马氏蝇Mar提取物中的八种生物活性成分，并将其用于药代动力学研究。

Marsdenia tenacissima（Roxb。）Wight et Arn作为中药。由于其在治疗恶性肿瘤，白细胞增多症，膀胱炎和哮喘中的特殊功效，在临床实践中起着不可或缺的作用。这项研究旨在建立一种新颖，科学的LC-MS / MS方法，同时测定大鼠血浆样品中的Tenacissima提取物中的Tenacissoside B，H，G和I，咖啡酸，隐绿原酸，绿原酸和新绿原酸。地高辛用作内部参考。所有测定均使用Eclipse Plus C _18进行色谱柱，水（含0.1％甲酸）用作流动相A，而乙腈是流动相B，用于梯度洗脱。根据生物药物分析的要求，对UPLC方法进行了验证，包括校准曲线，准确性，精密度，稳定性和全部八种分析物的回收率。此外，拟南芥提取物管饲后，该方法还用于大鼠八种分析物的综合药代动力学研究。根据药代动力学参数，tenacissoside B，I，H和G是tenacissima中的长效和主要生物活性成分提取物，具有长的平均停留时间和高浓度。我们的发现揭示了吸收机制，并为tenacissima的临床应用提供了重要信息。