This study shows that double thymidine block treatment efficiently arrests the EO771 cells in the S‐phase without altering cell growth or survival. A long‐term analysis of cell behavior, using 5(6)‐carboxyfluorescein diacetate N‐succinimidyl ester (CFSE) staining, show synchronization to be stable and consistent over time. The EO771 cell line is a medullary breast‐adenocarcinoma cell line isolated from a spontaneous murine mammary tumor, and can be used to generate murine tumor implantation models. Different biological (serum or amino acid deprivation), physical (elutriation, mitotic shake‐off), or chemical (colchicine, nocodazole, thymidine) treatments are widely used for cell synchronization. Of the different methods tested, the double thymidine block is the most efficient for synchronization of murine EO771 cells if a large quantity of highly synchronized cells is recommended to study functional and biochemical events occurring in specific points of cell cycle progression.

中文翻译：

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使用双胸苷块处理的鼠 EO771 细胞系的细胞周期同步。

该研究表明，双胸苷阻断治疗有效地将 EO771 细胞阻滞在 S 期，而不会改变细胞生长或存活。使用 5(6)-羧基荧光素二乙酸酯 N-琥珀酰亚胺酯 (CFSE) 染色对细胞行为进行的长期分析表明，随着时间的推移，同步是稳定和一致的。EO771 细胞系是从自发性鼠乳腺肿瘤中分离出来的髓质乳腺腺癌细胞系，可用于生成鼠肿瘤植入模型。不同的生物（血清或氨基酸剥夺）、物理（淘洗、有丝分裂抖落）或化学（秋水仙碱、诺考达唑、胸苷）治疗广泛用于细胞同步。在测试的不同方法中，