The transcription factor (TF) GATA2 plays a key role in organ development and cell fate control in the central nervous, urogenital, respiratory, and reproductive systems, and in primitive and definitive hematopoiesis. Here, we generate a knockin protein reporter mouse line expressing a GATA2VENUS fusion from the endogenous Gata2 genomic locus, with correct expression and localization of GATA2VENUS in different organs. GATA2VENUS expression is heterogeneous in different hematopoietic stem and progenitor cell populations (HSPCs), identifies functionally distinct subsets, and suggests a novel monocyte and mast cell lineage bifurcation point. GATA2 levels further correlate with proliferation and lineage outcome of hematopoietic progenitors. The GATA2VENUS mouse line improves the identification of specific live cell types during embryonic and adult development and will be crucial for analyzing GATA2 protein dynamics in TF networks.

转录因子（TF）GATA2在中枢神经，泌尿生殖系统，呼吸系统和生殖系统以及原始和确定的造血过程中的器官发育和细胞命运控制中起着关键作用。在这里，我们从内源性Gata2生成表达GATA2VENUS融合蛋白的敲入蛋白报告基因小鼠系基因座，在不同器官中正确表达和定位GATA2VENUS。GATA2VENUS表达在不同的造血干细胞和祖细胞群体（HSPCs）中是异质的，可识别功能上不同的亚群，并提示新的单核细胞和肥大细胞谱系分叉点。GATA2水平进一步与造血祖细胞的增殖和谱系结果相关。GATA2VENUS小鼠系改善了胚胎和成年发育过程中特定活细胞类型的识别，对于分析TF网络中的GATA2蛋白质动力学至关重要。