Abstract Hosta species endemic to Korea are currently suffering from infection with Hosta virus X (HVX), and it has been difficult to produce virus-free plants when using infected plants as a source of material for in vitro and vegetative propagation in continuous production processes. Genetic variation in in vitro-regenerated plants has also been detected in some Hosta cultivars. Therefore, we aimed to develop an in vitro propagation method for the production of virus-free and genetically stable plants. First, we collected Hosta capitata showing symptoms of viral infection and confirmed the presence of an HVX virus gene using reverse transcription (RT)–PCR, followed by optimization of the sterilization of in vivo bulbs. Meristems derived from sterilized bulbs were cultured in media containing different concentrations of 1-naphthaleneacetic acid (NAA) with either benzyl aminopurine (BA) or thidiazuron (TDZ). The combination of 0.1 mg/l NAA and 3.0 mg/l BA generated the highest number of shoots per explant, and these shoots had a higher fresh weight. The addition of nano-silver (NAg) particles to the regeneration medium distinctly stimulated plant growth. According to the results of RAPD analysis, the meristem-derived plants were genetically stable compared with donor plants grown in a greenhouse, and absence of the HVX gene was also observed, indicating freedom from HVX infection. Therefore, they would be highly valuable for use in the landscape industry. We expect that the method proposed in this study will also prove helpful for generating virus-free, genetically stable plants in the commercial production of other Hosta species.

中文翻译：

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建立分生组织培养，用于濒危植物玉簪的无病毒和遗传稳定生产

摘要 韩国特有的玉簪属目前正遭受玉簪病毒 X (HVX) 的感染，在连续生产过程中使用受感染的植物作为体外和营养繁殖的材料来源时，很难生产无病毒植物。在一些玉簪栽培品种中也检测到体外再生植物的遗传变异。因此，我们旨在开发一种用于生产无病毒且遗传稳定的植物的体外繁殖方法。首先，我们收集了表现出病毒感染症状的 Hosta capita，并使用逆转录 (RT)-PCR 确认了 HVX 病毒基因的存在，然后优化了体内球茎的灭菌。来自无菌球茎的分生组织在含有不同浓度的 1-萘乙酸 (NAA) 和苄基氨基嘌呤 (BA) 或噻二唑隆 (TDZ) 的培养基中培养。0.1 毫克/升 NAA 和 3.0 毫克/升 BA 的组合使每个外植体产生的枝条数量最多，并且这些枝条具有更高的鲜重。将纳米银 (NAg) 颗粒添加到再生培养基中明显刺激了植物生长。根据RAPD分析的结果，与温室中生长的供体植物相比，分生组织来源的植物在遗传上是稳定的，并且还观察到HVX基因的缺失，表明没有HVX感染。因此，它们对于景观行业的使用非常有价值。我们预计本研究中提出的方法也将证明有助于生成无病毒、