Blood-contacting devices including implantation devices, indwelling medical devices, and extracorporeal circuits have been at risk of thrombus and infections, which lead to treatment failure and serious adverse clinical events. In this study, we have reported a facile and robust mussel-inspired superhydrophilic coating for use on these blood-contacting devices to combat thrombosis and infections. The coating was fabricated mainly based on the rapid formation and accumulation of polydopamine (PDA) nanoparticles and in situ-synthesized using silver nanoparticles (Ag NPs) in the presence of sodium periodate. Therefore, the stability and long-term effects of superhydrophilicity were maintained because of the synergetic contribution of hydrophilic chemical components and surface topography of stacked nanoparticles. Different from mostly reported PDA coatings (with the water contact angle > 30°) that have good affinity to protein adsorption and cause blood coagulation, the superhydrophilic PDA coating in this work effectively reduced the nonspecific adsorption of proteins, maintained the native conformation of the adhered proteins, and further inhibited thrombus formation. Meanwhile, the superhydrophilic surface with Ag⁺ also demonstrated significant antibacterial properties against both Staphylococcus aureus (Gram-positive bacteria) and Pseudomonas aeruginosa (Gram-negative bacteria). Besides, the embedded Ag⁺ presented meaningful sustained release with less than 25% released amount even after 30 days incubation, suggesting effective and safe antibacterial ability in a low dose-dependent manner, due to the reduced protein adsorption platform supported killing and releasing of bacteria. Such a superhydrophilic surface-supported low-fouling platform will be proposed as a new strategy for combating thrombotic and infection, especially for blood-contacting devices.

包括植入设备，留置医疗设备和体外回路在内的血液接触设备有血栓和感染风险，这会导致治疗失败和严重的不良临床事件。在这项研究中，我们报道了一种轻便而坚固的贻贝类超亲水涂层，可用于这些与血液接触的设备，以对抗血栓形成和感染。主要根据聚多巴胺（PDA）纳米颗粒的快速形成和积累来制造涂层，并在高碘酸钠存在下使用银纳米颗粒（Ag NPs）原位合成。因此，由于亲水化学成分和堆叠纳米颗粒的表面形貌的协同作用，维持了超亲水性的稳定性和长期作用。与大多数报道的PDA涂层（水接触角> 30°）对蛋白质的吸附具有良好的亲和力并引起血液凝结不同，超亲水性PDA涂层在这项工作中有效地减少了蛋白质的非特异性吸附，保持了粘附的天然构象蛋白质，并进一步抑制血栓形成。同时，具有Ag的超亲水表面⁺还显示出对金黄色葡萄球菌（革兰氏阳性细菌）和铜绿假单胞菌（革兰氏阴性细菌）均具有显着的抗菌性能。此外，包埋的Ag ⁺甚至在孵育30天后仍表现出有意义的持续释放，释放量还不到25％，这表明它具有低剂量依赖性的有效和安全的抗菌能力，因为减少的蛋白质吸附平台支持细菌的杀死和释放。 。这种超亲水表面支撑的低污染平台将被提议作为对抗血栓形成和感染的新策略，特别是针对血液接触设备。