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Influence of sodium chloride on growth and metabolic reprogramming in nonprimed and haloprimed seedlings of blackgram (Vigna mungo L.)
Protoplasma ( IF 2.9 ) Pub Date : 2020-07-09 , DOI: 10.1007/s00709-020-01532-x
Sabarni Biswas 1, 2 , Asok K Biswas 1 , Bratati De 2
Affiliation  

Salinity hinders agricultural productivity worldwide by distressing plant metabolism. Growth of blackgram (Vigna mungo L. var. Sulata), an adverse climate-resistant pulse, is arrested under salinity. Present research integrates study of physio-biochemical parameters and non-targeted metabolomics approach to explore the alterations in metabolic pathway during adaptive responses of nonprimed and haloprimed blackgram seedlings grown hydroponically under NaCl stress. Salinity provoked accumulation of peroxides, compatible solutes and phenolics which increased free radical scavenging activities of nonprimed seedlings under salinity. Pre-germination seed halopriming abrogated NaCl-mediated adversities in haloprimed plantlets favouring better growth. Thus, farmers may adopt seed halopriming technique to improve blackgram productivity in saline-prone fields. Additionally, metabolomics study uncovered numerous metabolites amongst which 35 compounds altered significantly under salinity. The candidate metabolites were aspartic acid, l-glutamic acid, l-proline, l-asparagine, dl-isoleucine, l-homoserine, citrulline, l-ornithine, d-altrose, d-allose, N-acetyl-d-mannosamine, fructose, tagatose, sucrose, d-glucose, maltose, glycerol-1-phosphate, d-sorbitol, benzoic acid, shikimic acid, 4-hydroxycinnamic acid, arbutin, succinic acid, pipecolic acid, fumaric acid, nicotinic acid, l-pyroglutamic acid, oxalic acid, glyceric acid, maleamic acid, adenine, guanosine, lauric acid, stearic acid and porphine. Comparing metabolic responses of nonprimed and haloprimed seedlings, it was clear that efficient alteration in carbohydrate metabolism, phenolics accumulation, amino acid, organic acid and nucleic acid metabolism were the key places of metabolic reprogramming for tolerating salinity. Overall, we report, for the first time, 35 contributory candidate compounds that constituted core fundamental metabolome invoking salinity tolerance in nonprimed and haloprimed blackgram. These metabolites may be targeted by biotechnologists to produce high vigour salt-tolerant transgenic blackgram via genetic engineering.

中文翻译:

氯化钠对 blackgram (Vigna mungo L.) 未引物和卤引物幼苗生长和代谢重编程的影响

盐分会影响植物的新陈代谢,从而阻碍全世界的农业生产力。Blackgram (Vigna mungo L. var. Sulata) 是一种不利的耐气候性豆类,在盐度下会停止生长。目前的研究整合了生理生化参数和非靶向代谢组学方法的研究,以探索在 NaCl 胁迫下水培生长的非引物和卤引物黑格兰幼苗适应性反应过程中代谢途径的变化。盐度引起过氧化物、相容溶质和酚类物质的积累,从而增加盐度下未涂底漆幼苗的自由基清除活性。萌发前种子卤化引发消除了卤化引发的植株中 NaCl 介导的逆境,有利于更好的生长。因此,农民可以采用种子卤化引物技术来提高易盐碱地的黑格子产量。此外,代谢组学研究发现了许多代谢物,其中 35 种化合物在盐度下发生显着变化。候选代谢物是天冬氨酸、l-谷氨酸、l-脯氨酸、l-天冬酰胺、dl-异亮氨酸、l-高丝氨酸、瓜氨酸、l-鸟氨酸、d-altrose、d-阿洛糖、N-乙酰基-d-甘露糖胺、果糖、塔格糖、蔗糖、d-葡萄糖、麦芽糖、1-磷酸甘油、d-山梨糖醇、苯甲酸、莽草酸、4-羟基肉桂酸、熊果苷、琥珀酸、哌可酸、富马酸、烟酸、l-焦谷氨酸酸、草酸、甘油酸、马来酸、腺嘌呤、鸟苷、月桂酸、硬脂酸和卟啉。比较未引发剂和卤化剂幼苗的代谢反应,很明显,碳水化合物代谢、酚类物质积累、氨基酸、有机酸和核酸代谢的有效改变是耐受盐分的代谢重编程的关键场所。总的来说,我们首次报告了 35 种有贡献的候选化合物,它们构成了核心基础代谢组,在非引物和卤引物黑图中调用了耐盐性。这些代谢物可能会成为生物技术人员的目标,通过基因工程生产高活力耐盐转基因黑基因。35 种有贡献的候选化合物构成核心基本代谢组,在非引物和卤素黑图中调用耐盐性。这些代谢物可能会成为生物技术人员的目标,通过基因工程生产高活力耐盐转基因黑基因。35 种有贡献的候选化合物构成了核心基本代谢组,在非引物和卤素黑图中调用了耐盐性。这些代谢物可能会成为生物技术人员的目标,通过基因工程生产高活力耐盐转基因黑基因。
更新日期:2020-07-09
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