Anaplasmataceae includes the genera Anaplasma, Ehrlichia, Neorickettsia and Wolbachia, comprising a group of obligate intracellular bacteria. The genus Anaplasma has pathogenic species transmitted by ticks of veterinary and human health importance. Wild ungulates such as deer represent important reservoirs and amplifiers of Anaplasmataceae. The interaction between deer and domestic ruminants represents a serious problem due to the transmission of these pathogens through their ectoparasites. In the present study, we investigated the presence of Anaplasmataceae organisms in blood, tissues and tick samples of a gray-brocket deer (Mazama gouazoubira). The specimen was found dead in a farm in northeast Uruguay. PCRs targeting partial regions of 16S rRNA and groESL genes were carried out for Anaplasmataceae DNA detection. Moreover, several ectoparasites were identified: the chewing louse Tricholipeurus albimarginatus, the Neotropical deer louse fly Lipoptena mazamae, and the ticks Haemaphysalis juxtakochi and Rhipicephalus microplus. A consensus sequence of 1274 bp of 16S rRNA was generated for Anaplasma sp. from the M. gouazoubira blood sample. All ticks analysed by PCR assays were negative. No band was detected in any of the samples after PCR targeting groESL gene. Phylogenetic analysis using 16S rRNA partial gene sequences, clustered the putative novel genotype sequence obtained in this study, named Anaplasma sp. genotype Mazama—Uruguay, along with Anaplasma sp. detected in Mazama sp., Mazama americana and Mazama bororo, all deer species from Brazil. Furthermore, this cluster showed to be closely related to Anaplasma bovis sequences obtained from various ruminants and other mammals from several parts of the world. The pathogenicity as well as its infecting potential to other cervids or domestic ruminants is currently unknown. Further studies should be performed in order to characterize this novel species, especially targeting other genes.

中文翻译：

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在乌拉圭的灰de鹿（Mazama gouazoubira）中检测到一种推定的无形体基因型。

Anaplasmataceae包括Anaplasma，Ehrlichia，Neorickettsia和Wolbachia属，包括一组专性的细胞内细菌。Anaplasma属具有通过兽医和人类健康重要性tick传播的病原体。诸如鹿之类的野生有蹄类动物代表了无形纲科的重要储藏和繁殖体。鹿与反刍动物之间的相互作用是一个严重的问题，因为这些病原体通过其体外寄生虫传播。在本研究中，我们调查了灰-鹿（Mazama gouazoubira）的血液，组织和壁虱样本中无形纲细菌的存在。该标本被发现死于乌拉圭东北部的一个农场。进行了针对16S rRNA和groESL基因的部分区域的PCR，以检测无性科的DNA。此外，还鉴定出几种外寄生虫：咀嚼虱子Tricholipeurus albimarginatus，新热带鹿虱蝇Lipoptena mazamae和s虫Haemaphysalis juxtakochi和Rhipicephalus microplus。产生了Anaplasma sp。的1274 bp的16S rRNA的共有序列。从gouazoubira M.血样中提取。通过PCR测定分析的所有壁虱均为阴性。PCR靶向groESL后在任何样品中均未检测到条带基因。使用16S rRNA部分基因序列进行系统发育分析，将本研究中获得的推定的新基因型序列聚类为Anaplasma sp。基因型Mazama-Uruguay，以及Anaplasma sp。在巴西的Mazama sp。，Mazama americana和Mazama bororo中检测到。此外，该簇与牛无形体密切相关从世界各地的各种反刍动物和其他哺乳动物获得的序列。目前尚不清楚其致病性及其对其他子宫颈或反刍动物的感染潜力。为了表征这种新物种，应该进行进一步的研究，尤其是针对其他基因。