The aim was the evaluation of IL-6 concentration in peritoneal lavage fluid of children which underwent cholecystectomy to ascertain if there is a difference in early inflammatory response depending on the type of surgical approach (open vs. laparoscopy). The analysis of high-mobility group protein B1 (HMGB1) and heat shock protein 70 (HSP70) was performed to find out if the source of IL-6 was related to tissue damage. IL-6 concentration in peritoneal lavage fluid samples, obtained at the beginning and at the end of the laparoscopic () and open cholecystectomy (), was tested with a routinely used electrochemiluminescence assay. The concentrations of HMGB1 and HSP70 were analyzed with the use of an ELISA method. Statistical analysis was performed using the STATISTICA PL release 12.5 Program. The differences were assessed using the Mann-Whitney test and Wilcoxon matched pairs test. Correlations were studied by using the Spearman correlation test. Our results demonstrated significant peritoneal lavage fluid IL-6 concentration growth measured at the end of the cholecystectomy as compared to the beginning, regardless of the type of the procedure. IL-6 growth during open cholecystectomy was greater compared to laparoscopic cholecystectomy (62.51-fold vs. 3.19-fold). IL-6 concentration did not correlate with HMGB1 and HSP70, which indicate that the significant growth of this cytokine was not related to mechanical tissue damage due to surgical procedure. A clinical significance of the study could be related to the fact that the evaluation of IL-6 concentration in peritoneal lavage fluid may be useful to assess an early local inflammatory response.

中文翻译：

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进行胆囊切除术的儿童术中腹膜白细胞介素6浓度变化与高迁移率族蛋白B1和热休克蛋白70水平的关系。

目的是评估接受胆囊切除术的儿童的腹腔灌洗液中的IL-6浓度，以确定早期炎症反应是否存在差异，具体取决于手术方法的类型（开放式与腹腔镜检查）。对高迁移率族蛋白B1（HMGB1）和热休克蛋白70（HSP70）进行了分析，以找出IL-6的来源是否与组织损伤有关。在腹腔镜开始和结束时获得的腹腔灌洗液样品中的IL-6浓度（）和开腹胆囊切除术（），用常规使用的电化学发光测定中测试。使用ELISA方法分析HMGB1和HSP70的浓度。使用STATISTICA PL 12.5版程序进行统计分析。使用Mann-Whitney评估差异测试和Wilcoxon配对测试。通过使用Spearman相关检验研究相关性。我们的结果表明，与手术开始时相比，在胆囊切除术结束时测得的腹腔灌洗液IL-6浓度明显增加，而与手术类型无关。与腹腔镜胆囊切除术相比，开腹胆囊切除术中IL-6的生长更大（62.51倍对3.19倍）。IL-6的浓度与HMGB1和HSP70不相关，这表明该细胞因子的显着增长与外科手术引起的机械组织损伤无关。该研究的临床意义可能与以下事实有关：评估腹腔灌洗液中IL-6的浓度可能有助于评估早期的局部炎症反应。