当前位置: X-MOL 学术Zygote › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Maternal ageing causes changes in DNA methylation and gene expression profiles in mouse oocytes
Zygote ( IF 1.7 ) Pub Date : 2020-07-08 , DOI: 10.1017/s0967199420000143
Guanmei Hou 1, 2 , Qing-Yuan Sun 1, 2
Affiliation  

SummaryAlthough it is well known that maternal ageing causes reduced oocyte quality and fertility, little information is known about its effect on germ cell epigenetics. In the present study, we compared the gene expression and DNA methylation profiles in germinal vesicle oocytes from young (8-week-old) and aged (18-month-old) mice using single-cell RNA-sequencing and single-cell whole-genome DNA methylation sequencing. We found significant differences in the data from the two groups. Oocytes from aged mice showed significant changes in the expression of some metabolism-related genes, such as mitochondria-associated genes, that was in line with our expectations. Expression of some genes associated with reproduction also showed significant differences. DNA methylation levels were also changed in oocytes from aged mice. The two groups had significant gaps in hypermethylation and hypomethylation levels on each chromosome. These data provide useful information for further understanding the mechanisms of oocyte ageing.

中文翻译:

母体衰老导致小鼠卵母细胞 DNA 甲基化和基因表达谱的变化

摘要虽然众所周知,母体衰老会导致卵母细胞质量和生育能力下降,但关于其对生殖细胞表观遗传学的影响的信息知之甚少。在本研究中,我们使用单细胞 RNA 测序和单细胞全细胞比较年轻(8 周大)和老年(18 个月大)小鼠的生泡卵母细胞中的基因表达和 DNA 甲基化谱。基因组DNA甲基化测序。我们发现两组的数据存在显着差异。来自老年小鼠的卵母细胞显示出一些代谢相关基因的表达发生了显着变化,例如线粒体相关基因,这符合我们的预期。一些与繁殖相关的基因的表达也表现出显着差异。来自老年小鼠的卵母细胞中的 DNA 甲基化水平也发生了变化。两组在每条染色体上的高甲基化和低甲基化水平存在显着差异。这些数据为进一步了解卵母细胞衰老的机制提供了有用的信息。
更新日期:2020-07-08
down
wechat
bug