As surfaces for enzyme immobilization, lipid bilayers (LBs) have the potential to exhibit chaperone‐like activity, which can greatly enhance enzyme stability. Here, it is shown that this stabilizing effect can be generalized to a broad range of enzymes by modifying the mixed lipid composition based on the properties of the enzyme. This is demonstrated by analyzing the stability of nitroreductase, lipase, organophosphorus hydrolase, and lysozyme on LBs composed by mixing zwitterionic 1,2‐dioleoyl‐sn‐glycero‐3‐phosphocholine with anionic 1,2‐dioleoyl‐sn‐glycero‐3‐phospho‐(1′‐rac‐glycerol) or cationic 1,2‐dioleoyl‐sn‐glycero‐3‐ethylphosphocholine. Interestingly, while the optimal lipid composition exhibits a zeta potential with the same sign as each enzyme, the stabilizing compositional ranges are broad, and mixed lipid LBs are more stabilizing than homogeneous LBs with similar charge. This suggests that net charge and compositional heterogeneity are both important factors. Moreover, the degree of stabilization on appropriate bilayers under extremely denaturing conditions (7 m urea) is unprecedented, and activity of the enzymes is up to three‐fold greater than that of the soluble enzyme at each enzyme's temperature optimum. By correlating enzyme diffusion on LBs with information about re‐folding kinetics derived from single‐molecule measurements, the influence of charge and composition on enzyme‐LB interactions is shown, providing a rationale for the stabilizing effect of heterogeneous LBs.

中文翻译：

---

工程化异构脂质双层的组成，以稳定拴系酶。

作为固定酶的表面，脂质双层（LBs）可能表现出类似伴侣的活性，这可以大大增强酶的稳定性。在此表明，通过基于酶的性质改变混合脂质组成，可以将这种稳定作用推广到广泛的酶中。这是通过分析两性离子1,2-油酰基-sn-甘油-3-磷酸胆碱与阴离子1,2-油酰基-sn-甘油-3-混合组成的LB上的硝基还原酶，脂肪酶，有机磷水解酶和溶菌酶的稳定性来证明的。磷酸（1'-rac-甘油）或阳离子1,2-二油酰基-sn-甘油-3-乙基磷酸胆碱。有趣的是，虽然最佳脂质成分的Zeta电位与每种酶的符号相同，但稳定成分的范围很广，和混合脂质LB相比具有相似电荷的均质LB更稳定。这表明净电荷和组成异质性都是重要因素。此外，在极度变性的条件下，适当双层膜的稳定程度（7m尿素）是前所未有的，并且在每种酶的最佳温度下，酶的活性比可溶性酶的活性高三倍。通过将酶在LB上的扩散与从单分子测量得出的重折叠动力学信息相关联，可以显示电荷和组成对酶与LB相互作用的影响，从而为异质LB的稳定作用提供了理论依据。