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A nuclear protein NsiA from Epichloë festucae interacts with a MAP kinase MpkB and regulates the expression of genes required for symbiotic infection and hyphal cell fusion.
Molecular Microbiology ( IF 3.6 ) Pub Date : 2020-07-07 , DOI: 10.1111/mmi.14568
Aiko Tanaka 1, 2 , Shota Kamiya 1 , Yoshino Ozaki 1 , Shinichi Kameoka 1 , Yuka Kayano 1 , Sanjay Saikia 2 , Fumitake Akano 1 , Aiko Uemura 3 , Hiroki Takagi 3 , Ryohei Terauchi 3 , Jun-Ichi Maruyama 4 , Hamzeh Haj Hammadeh 5 , André Fleissner 5 , Barry Scott 2 , Daigo Takemoto 1, 2
Affiliation  

The endophytic fungus Epichloë festucae systemically colonizes the intercellular spaces of cool‐season grasses to establish a mutualistic symbiosis. Hyphal growth of the endophyte within the host plant is tightly regulated and synchronized with the growth of the host plant. A genetic screen to identify symbiotic genes identified mutant FR405 that had an antagonistic interaction with the host plant. Perennial ryegrass infected with the FR405 mutant were stunted and underwent premature senescence and death. The disrupted gene in FR405 encodes a nuclear‐localized protein, designated as NsiA for nuclear protein for symbiotic infection. Like previously isolated symbiotic mutants the nsiA mutant is defective in hyphal cell fusion. NsiA interacts with Ste12, a C2H2 zinc‐finger transcription factor, and a MAP kinase MpkB. Both are known as essential components for cell fusion in other fungal species. In E. festucae, MpkB, but not Ste12, is essential for cell fusion. Expression of several genes required for cell fusion and symbiosis, including proA/adv‐1, pro41/ham‐6, ham7, ham8, and ham9 were downregulated in the nsiA mutant. However, the NsiA ortholog in Neurospora crassa was not essential for hyphal cell fusion. These results demonstrate that the roles of NsiA and Ste12 orthologs in hyphal cell fusion are distinctive between fungal species.

中文翻译:

上蜡EpiEpichloëfestucae的核蛋白NsiA与MAP激酶MpkB相互作用,并调节共生感染和菌丝细胞融合所需的基因表达。

内生真菌Epichloëfestucae系统地定居在凉季草的细胞间空间,建立了一种共生的共生关系。宿主植物内生菌的菌丝生长受到严格调节,并与宿主植物的生长同步。鉴定共生基因的遗传筛选鉴定出与宿主植物具有拮抗作用的突变体FR405。感染了FR405突变体的多年生黑麦草发育迟缓,并过早衰老和死亡。FR405中的破坏基因编码一种核定位蛋白,称为共生感染核蛋白NsiA。像以前分离的共生突变体,nsiA突变体在菌丝细胞融合中有缺陷。NsiA与Ste12,C2H2锌指转录因子和MAP激酶MpkB相互作用。两者均被称为其他真菌物种中细胞融合的必要成分。在羊茅中,MpkB而不是Ste12对于细胞融合是必不可少的。在nsiA突变体中,细胞融合和共生所需的几种基因,包括proA / adv-1pro41 / ham-6ham7,ham8ham9的表达下调。然而,神经孢霉中的NsiA直向同源物对于菌丝细胞融合不是必需的。这些结果表明,NsiA和Ste12直向同源物在菌丝细胞融合中的作用在真菌种类之间是独特的。
更新日期:2020-07-07
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