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Associations between clinical features and therapy with macrophage subpopulations and T cells in inflammatory lesions in the aorta from patients with Takayasu arteritis.
Clinical & Experimental Immunology ( IF 4.6 ) Pub Date : 2020-07-08 , DOI: 10.1111/cei.13489
J P Dos Santos 1 , R Artigiani Neto 2 , C L P Mangueira 3 , R Z Filippi 4 , P S Gutierrez 5 , J Westra 6 , E Brouwer 6 , A W S de Souza 1
Affiliation  

Takayasu arteritis (TAK) is a large‐vessel granulomatous vasculitis; the inflammatory infiltration in arteries comprises macrophages, multi‐nucleated giant cells, CD4+ and CD8+ T cells, γδ T cells, natural killer (NK) cells and neutrophils. However, it is unknown which subtype of macrophages predominates. This study aims to evaluate macrophages subpopulations in the aorta in TAK. Immunohistochemistry was performed in the aorta from TAK patients (n = 22), patients with atherosclerotic disease (n = 9) and heart transplant donors (n = 8) using the markers CD68, CD86, CD206, CD3, CD20 and CD56. Active disease was observed in 54·5% of patients and active histological lesions were found in 40·9%. TAK patients presented atherosclerotic lesions in 27·3% of cases. The frequency of macrophages, M1 macrophages, T, B and NK cells was higher in the aorta from TAK and atherosclerotic patients compared to heart transplant donors. In TAK, macrophages and T cells were the most abundant cells in the aorta, and the expression of CD206 was higher than CD86 (P = 0·0007). No associations were found between the expression of cell markers and active disease or with atherosclerotic lesions. In TAK patients, histological disease activity led to higher T cell counts than chronic fibrotic lesions (P = 0.030), whereas prednisone use was associated with lower T cell counts (P = 0·035). In conclusion, M1 macrophages were more frequent in TAK and atherosclerotic patients compared to heart transplant donors, while M2 macrophages dominated M1 macrophages in TAK. T cells were associated with histological disease activity and with prednisone use in TAK.

中文翻译:

Takayasu 动脉炎患者主动脉炎性病变中的临床特征与巨噬细胞亚群和 T 细胞治疗之间的关联。

Takayasu 动脉炎(TAK)是一种大血管肉芽肿性血管炎;动脉炎症浸润包括巨噬细胞、多核巨细胞、CD4 +和CD8 + T细胞、γδT细胞、自然杀伤(NK)细胞和中性粒细胞。然而,尚不清楚哪种巨噬细胞亚型占主导地位。本研究旨在评估 TAK 主动脉中的巨噬细胞亚群。在 TAK 患者 ( n  = 22)、动脉粥样硬化疾病患者 ( n  = 9) 和心脏移植供体 ( n = 8) 使用标记 CD68、CD86、CD206、CD3、CD20 和 CD56。54·5% 的患者观察到活​​动性病变,40·9% 的患者发现活动性组织学病变。27·3% 的 TAK 患者出现动脉粥样硬化病变。与心脏移植供者相比,来自 TAK 和动脉粥样硬化患者的主动脉中巨噬细胞、M1 巨噬细胞、T、B 和 NK 细胞的频率更高。在TAK中,巨噬细胞和T细胞是主动脉中含量最多的细胞,CD206的表达高于CD86(P  = 0·0007)。未发现细胞标志物的表达与活动性疾病或动脉粥样硬化病变之间存在关联。在 TAK 患者中,组织学疾病活动导致 T 细胞计数高于慢性纤维化病变(P = 0.030),而使用泼尼松与较低的 T 细胞计数相关 ( P  = 0·035)。总之,与心脏移植供体相比,TAK 和动脉粥样硬化患者中 M1 巨噬细胞的频率更高,而 M2 巨噬细胞在 TAK 中以 M1 巨噬细胞为主。T 细胞与组织学疾病活动和 TAK 中泼尼松的使用有关。
更新日期:2020-07-08
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