LncRNA LINC‐PINT acts as an important regulator in the development of many cancers. The current study aimed to explore the role of LINC‐PINT in the progression of ovarian cancer (OC). LINC‐PINT expression level in different FIGO stages of OC and its adjacent tissues, normal HOSE and OC cell lines (A2780, SKOV3, OVCAR3 and HO‐8910) was determined by qRT‐PCR. Survival analysis on LINC‐PINT and OC patients was conducted by Kaplan‐Meier. CCK‐8, flow cytometry, wound‐healing, Transwell assays and western blot were performed to detect the effects of LINC‐PINT on proliferation, apoptosis, migration, invasion and EMT process in OC cells. Target gene of LINC‐PINT was predicted by Starbase and verified by dual‐luciferase reporter assay. The expression of miR‐374a‐5p in normal and OC tissues, LINC‐PINT‐ or siLINC‐PINT‐modified OC cells was determined. Moreover, rescue assay was carried out to confirm whether LINC‐PINT contributes to the development of OC cells through targeting miR‐374a‐5p. Low expression of LINC‐PINT was observed in OC tissues and cells, noticeably, LINC‐PINT expression was even lower in OC tissues with higher FIGO stage. Increased LINC‐PINT expression significantly inhibited cell proliferation, promoted apoptosis and suppressed migration, invasion and EMT process, while silencing of LINC‐PINT caused the opposite results. Moreover, LINC‐PINT sponged miR‐374a‐5p and overexpressed miR‐374a‐5p attenuated the effect of up‐regulated LINC‐PINT on cell viability, migration, invasion and apoptosis. LINC‐PINT acts as a tumour suppressor, as it could inhibit cell proliferation, migration, invasion and EMT process, and promote cell apoptosis through down‐regulating miR‐374a‐5p.

中文翻译：

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LINC-PINT通过靶向miR-374a-5p在卵巢癌中抑制肿瘤细胞的增殖，迁移和侵袭。

LncRNA LINC-PINT在许多癌症的发生中起着重要的调节作用。本研究旨在探讨LINC-PINT在卵巢癌（OC）进展中的作用。通过qRT-PCR测定了OC及其邻近组织，正常HOSE和OC细胞系（A2780，SKOV3，OVCAR3和HO-8910）在FIGO不同阶段的LINC-PINT表达水平。Kaplan-Meier对LINC-PINT和OC患者进行了生存分析。进行CCK-8，流式细胞术，伤口愈合，Transwell分析和蛋白质印迹以检测LINC-PINT对OC细胞增殖，凋亡，迁移，侵袭和EMT过程的影响。LINC-PINT的靶基因由Starbase预测，并通过双荧光素酶报告基因检测法验证。确定了正常和OC组织，LINC-PINT或si LINC-PINT修饰的OC细胞中miR-374a-5p的表达。此外，进行了拯救试验以确认LINC-PINT是否通过靶向miR-374a-5p促进OC细胞的发育。在OC组织和细胞中观察到LINC-PINT的低表达，值得注意的是，在FIGO期较高的OC组织中，LINC-PINT的表达甚至更低。LINC-PINT表达增加可显着抑制细胞增殖，促进细胞凋亡并抑制迁移，侵袭和EMT过程，同时使LINC-PINT沉默导致相反的结果。而且，LINC‐PINT可以使miR‐374a‐5p海绵化并过度表达miR‐374a‐5p，从而减弱了LINC‐PINT上调对细胞活力，迁移，侵袭和凋亡的影响。LINC‐PINT可以抑制细胞增殖，迁移，侵袭和EMT过程，并通过下调miR‐374a‐5p促进细胞凋亡，从而起到抑癌作用。