Transcriptome analysis of Clarias magur brain and gonads at preparatory, mature, 6 and 16 h post-GnRH injection (hpi) stages yielded 9.5 GB data with 39,738 contigs. Sequences of 45 reproductive genes were identified for the first time in C. magur along with unique and differentially expressed genes. The expression of 20 genes was validated by qRT-PCR. Upregulation of Cyp11A1, Cyp17A1 and FTZF1 genes in the 16hpi testis accompanied by the 17β-HSD3 expression indicates testosterone (T) synthesis in response to LH surge, while reduced expression of CYP11B1 suggests a high T: 11-KT ratio. It is evident by the gene expression analysis that the inhibitory neurotransmitter GABA, altered T: 11-KT, increased testicular bile acids, and oxytocin-like neuropeptide in the male brain, appear to be involved in arresting the pulsatile motion of testicular smooth muscles. The work generates important leads for an effective induced breeding strategy for silurid catfish.

在准备、成熟、GnRH 注射 (hpi) 后 6 和 16 小时阶段对Clarias magur大脑和性腺的转录组分析产生了 9.5 GB 数据和 39,738 个重叠群。首次在C. magur中鉴定出45个生殖基因序列以及独特和差异表达的基因。通过qRT-PCR验证了20个基因的表达。伴随 17β-HSD3 表达的 16hpi 睾丸中 Cyp11A1、Cyp17A1 和 FTZF1 基因的上调表明睾酮 (T) 合成响应 LH 激增，而 CYP11B1 的表达降低表明 T: 11-KT 比率较高。基因表达分析表明，抑制性神经递质 GABA、T: 11-KT 改变、睾丸胆汁酸增加和男性大脑中的催产素样神经肽似乎参与阻止睾丸平滑肌的搏动运动。这项工作为鲶鱼的有效诱导育种策略提供了重要线索。