The Revogene® Carba C assay is a real-time polymerase chain reaction–based assay that runs on the microfluidic Revogene platform. It was recently designed for the detection of genes encoding the 5 major carbapenemases (NDM, VIM, IMP, KPC, and OXA-48) from various Gram-negatives. A total of 145 clinical Gram-negative strains (96 carbapenemase producers and 49 non–carbapenemase producers) were tested. The overall sensitivity and specificity were 100%. All strains co-producing double carbapenemases have been correctly detected. All non–carbapenemase producers and nontargeted carbapenemase producers gave a negative result. The sample preparation was easy to handle, taking around 5 to 10 min per isolate, with a run time of approximately 70 min. This assay is a rapid, easy-to-perform, reliable tool to detect the most common carbapenemases, with excellent sensitivity and specificity regardless of the host bacteria. Given its user friendliness, simplicity, and short time to result, the Revogene® Carba C assay is suitable for microbiology laboratories.

Revogene®Carba C分析是基于实时聚合酶链反应的分析，可在微流体Revogene平台上运行。最近，它被设计用于检测来自各种革兰氏阴性菌的5种主要碳青霉烯酶（NDM，VIM，IMP，KPC和OXA-48）的基因。总共测试了145株临床革兰氏阴性菌株（96个碳青霉烯酶生产者和49个非卡培南酶生产者）。总体敏感性和特异性为100％。已正确检测到所有共同产生双碳青霉烯酶的菌株。所有非卡巴内酶生产商和非靶向碳青霉烯酶生产商均给出了阴性结果。样品制备操作简便，每个分离株大约需要5至10分钟，运行时间约为70分钟。该测定法是一种快速，易于执行且可靠的工具，可检测出最常见的碳青霉烯酶，无论宿主细菌是什么，都具有出色的灵敏度和特异性。Revogene®Carba C分析方法具有用户友好性，简便性和较短的结果生成时间，因此适用于微生物实验室。