Herein, some (modified) paper-based substrates were prepared and utilized as extractive phases in a microfluidic device and their extraction performances examined for analytes with different polarities. Reagents including hexadecyltrimethoxysilane (HDTMS), phenyltrimethoxysilane (PTES), (3-aminopropyl) triethoxysilane (APTES) and 3-(2,3-epoxypropoxy) propyltrimethoxysilane (EPPTMOS) were implemented for the modification process. Due to the induction of different silane functional groups, it was anticipated to have various interactions for the tested analytes. Eventually, the prepared paper sheets were used as extractive phases for solid-phase extraction within a microfluidic system. The microfluidic setup not only improves the mechanical stability of the paper-based phases but also facilitated the mass transfer process and decreases the energy consumption. The selected analytes contained antidepressant drugs, organophosphates and triazine toxins, having different structures and polarities. Afterward, the existing interactions between the paper-based sorbents and the selected analytes were investigated by Principal Component Analysis (PCA) and Hierarchical Clustering Analysis (HCA) for data analysis. According to the obtained data, the PTES modified surface paper was selected for quantitative investigation and optimization. The major parameters associated with the extraction performance were studied. A linear range was obtained for amitriptyline and trimipramine, 0.05-200 μg L-1, and the calibration graph for clomipramine was linear in the range of 0.02-200 μg L-1. The employment of microfluidic device in conjunction with gas chromatography-mass spectrometry led to the limits of detections of 0.005-0.01 μgL-1 for the antidepressant drugs. The relative recoveries for the tested drugs in urine samples were in the range of 95-103%.

中文翻译：

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基于硅烷的改性纸及其在微流体平台中的萃取相作用

在此，制备了一些（改性的）纸基基材并将其用作微流体装置中的提取相，并检查了它们对不同极性分析物的提取性能。包括十六烷基三甲氧基硅烷 (HDTMS)、苯基三甲氧基硅烷 (PTES)、(3-氨基丙基) 三乙氧基硅烷 (APTES) 和 3-(2,3-环氧丙氧基) 丙基三甲氧基硅烷 (EPPTMOS) 在内的试剂用于改性过程。由于不同硅烷官能团的诱导作用，预计被测分析物会发生各种相互作用。最终，制备的纸张被用作微流体系统内固相萃取的萃取相。微流体装置不仅提高了纸基相的机械稳定性，而且促进了传质过程并降低了能耗。所选分析物包含抗抑郁药物、有机磷酸盐和三嗪毒素，具有不同的结构和极性。然后，通过主成分分析 (PCA) 和层次聚类分析 (HCA) 对纸基吸附剂与所选分析物之间现有的相互作用进行研究，以进行数据分析。根据获得的数据，选择PTES改性表面纸进行定量研究和优化。研究了与提取性能相关的主要参数。阿米替林和曲米帕明的线性范围为 0.05-200 μg L-1，氯米帕明的校准曲线在 0.02-200 μg L-1 范围内呈线性。微流体装置与气相色谱-质谱联用导致抗抑郁药物的检测限为 0.005-0.01 μgL-1。尿样中受试药物的相对回收率在 95-103% 的范围内。