V. minor contains monomeric eburnamine-type of indole alkaloids having utilization as a neuro-medicinal plant. The biosynthetic pathway studies using miRNAs has been the focal point for plant genomic research in recent years and this technique is utilized to get an insight into a possible pathway level study in V. minor as understanding of genes in this prized medicinal plant is meagrely understood. The de novo transcriptomic analysis using Illumina Next gen sequencing has been performed in glasshouse shifted plant and transformed roots to elucidate the possible non confirmed steps of terpenoid indole alkaloids (TIAs) pathway in V. minor. A putative TIA pathway is elucidated in the study including twelve possible TIAs biosynthetic genes. The specific miRNA associated with TIAs pathway were identified and their roles were discussed for the first time in V. minor. The comparative analysis of transcriptomic data of glasshouse shifted plant and transformed roots showed that the raw reads of transformed roots were higher (83,740,316) compared to glasshouse shifted plant (67,733,538). The EST-SSR prediction showed the maximum common repeats among glasshouse shifted plant and transformed roots, although small variation was found in trinucleotide repeats restricted to glasshouse shifted plant. The study reveals overall 37 miRNAs which were observed to be true and can have a role in pathway as they can regulate the growth and alkaloid production. The identification of putative pathway genes plays an important role in establishing linkage between Aspidosperma and Eburnamine alkaloids.

中文翻译：

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小长春花中的 TIAs 通路基因和相关 miRNA 鉴定：通过转录组学分析支持有子植物和 eburnamine 生物碱的联系。

V. minor含有单体 eburnamine 型吲哚生物碱，可用作神经药用植物。近年来，使用 miRNA 的生物合成途径研究一直是植物基因组研究的焦点，并且由于对这种珍贵药用植物中基因的理解知之甚少，因此该技术被用于深入了解小葡萄球菌中可能的途径水平研究。使用 Illumina Next gen 测序的从头转录组学分析已在温室转移植物和转化根中进行，以阐明小葡萄中萜类吲哚生物碱 (TIA) 途径的可能未确认步骤. 该研究阐明了一个推定的 TIA 途径，包括十二个可能的 TIA 生物合成基因。鉴定了与 TIAs 通路相关的特定 miRNA 并首次在V. minor中讨论了它们的作用. 温室转移植物和转化根的转录组数据的比较分析表明，与温室转移植物 (67,733,538) 相比，转化根的原始读数更高 (83,740,316)。EST-SSR 预测显示温室转移植物和转化根之间的最大共同重复，尽管在仅限于温室转移植物的三核苷酸重复中发现了小的变异。该研究揭示了总共 37 种 miRNA，它们被观察到是真实的，并且可以在途径中发挥作用，因为它们可以调节生长和生物碱的产生。推定通路基因的鉴定在建立曲霉属和 Eburnamine 生物碱之间的联系方面起着重要作用。