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Label-free and self-assembled fluorescent DNA nanopompom for determination of miRNA-21
Microchimica Acta ( IF 5.7 ) Pub Date : 2020-07-07 , DOI: 10.1007/s00604-020-04377-6
Nandi Chen 1 , Junyu Li 1 , Xianzhen Feng 1 , Yanping Yang 2 , Li Zhu 1 , Xiaomeng Chen 1 , Xuan Liu 1 , Yang Li 1 , Cunchuan Wang 3 , Ligang Xia 1
Affiliation  

A label-free fluorescence method based on self-assembled DNA nanopompom has been developed for miRNA-21 detection. In the presence of miRNA-21, three DNA hairpin probes with split G-quadruplex assemble the DNA nanopompom. Based on the isothermal toehold-mediated DNA strand displacement reaction, the target miRNA can be catalytically recycled and trigger three DNA hairpin probes to self-assemble the DNA nanopompom and release the G-quadruplex. The formation of the G-quadruplex increases the fluorescence emission intensity of thioflavin. For thioflavin-based miRNA-21 detection, the excitation and emission wavelengths are set to 425 nm and 490 nm, respectively. The limit of detection for miRNA-21 is 0.8 pM according to F / F 0 = 0.0031 × C miRNA-21 + 1.0382 ( R 2 = 0.9978). This sensing system provides a low-cost, effective, and convenient method for miRNA detection, which holds great potential in biochemical diagnosis and clinical practice. Graphical abstract Label-free and self-assembled fluorescent DNA nanopompom for miRNA detection

中文翻译:

用于测定 miRNA-21 的无标记和自组装荧光 DNA 纳米绒球

一种基于自组装 DNA 纳米绒球的无标记荧光方法已被开发用于 miRNA-21 检测。在存在 miRNA-21 的情况下,三个带有分裂 G-四链体的 DNA 发夹探针组装 DNA 纳米绒球。基于等温立足点介导的 DNA 链置换反应,目标 miRNA 可以催化回收并触发三个 DNA 发夹探针自组装 DNA 纳米绒球并释放 G-四链体。G-四链体的形成增加了硫代黄素的荧光发射强度。对于基于硫黄素的 miRNA-21 检测,激发和发射波长分别设置为 425 nm 和 490 nm。根据 F / F 0 = 0.0031 × C miRNA-21 + 1.0382 (R 2 = 0.9978),miRNA-21 的检测限为 0.8 pM。这种传感系统提供了一种低成本、有效、miRNA检测的简便方法,在生化诊断和临床实践中具有巨大的潜力。用于 miRNA 检测的无标记和自组装荧光 DNA 纳米绒球的图形摘要
更新日期:2020-07-07
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