Pseudotrachydium kotschyi is a native medicinal plant used as a local medicine from Lorestan province of western Iran having numerous biological properties. After GC−MS analysis of Pseudotrachydium kotschyi essential oils (PKEOs), some biological activities were investigated. Additionally, TNF-α and TGF-β1 were studied in Leishmania-infected macrophages (in vitro) and Carrageenan-induced paw edema in rats. Finally, anti-inflammatory and wound-healing potential of PKEOs were investigated in vivo in mice. Total components identified by GC−MS contained 53 molecules, among which Z-α-trans-Bergamotol (23.25%), Durylaldehyde (16.07%), and α-Bergamotene (10.48%) existed in the highest amount in PKEOs. Antimicrobial assay of PKEOs showed maximum growth inhibition against Listeria monocytogenes and Kluyveromyces marxianus at the dose of 625 μg/ml. PKEOs exhibited a high cytotoxicity on lung cancer cell line A549 at a dose of 4 mg/ml. Anti-Leishmania activity of PKEOs showed concentration of 5000 µg/ml that had similar potency of glucantime at 125 µg/ml. The highest anti-inflammatory effect of PKEOs for Carrageenan-induced paw edema was observed at 100 concentrations, with paw inflammation reduced by 83.70% after using PKEO ointment. The present study reports broad-spectrum biological functions for essential oil from P. kotschyi. However, these biological activities, including anti-proliferative, anti-Leishmania, anti-inflammatory, antimicrobial and wound-healing potential, were not studied in detail. Therefore, it may be attractive for investigating the detailed mechanistic and molecular aspects of biological activities of PKEOs. Considering that the present study is the first to report the biological activity of P. kotschyi, further studies on their metabolites are needed to replicate the present findings.

中文翻译：

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伪太子参精油的植物化学组成鉴定和生物活性评估

Pseudotrachydium kotschyi是一种天然药用植物，被用作伊朗西部Lorestan省的一种当地药物，具有许多生物学特性。之后的GC-MS分析Pseudotrachydium kotschyi精油（PKEOs），一些生物活性进行了调查。另外，在利什曼原虫感染的巨噬细胞（体外）和角叉菜胶诱导的足爪水肿中研究了TNF-α和TGF-β1 。最后，在小鼠体内研究了PKEOs的抗炎和伤口愈合潜力。GC-MS鉴定的总组分包含53个分子，其中Z-α-反式-Bergamotol（23.25％），Durylaldehyde（16.07％）和α-Bergamotene（10.48％）在PKEO中的含量最高。PKEOs的抗菌检测显示对单核细胞增生李斯特菌和马克斯克鲁维酵母的剂量为625μg / ml。PKEOs以4 mg / ml的剂量对肺癌细胞A549表现出高细胞毒性。PKEOs的抗利什曼原虫活性显示浓度为5000 µg / ml，与125μg/ ml的葡聚糖时效相似。在100浓度下，PKEO对角叉菜胶诱发的爪水肿具有最高的抗炎作用，使用PKEO软膏后，爪炎减少了83.70％。本研究报告了P. kotschyi精油的广谱生物学功能。但是，这些生物活性包括抗增殖，抗利什曼原虫抗炎，抗菌和伤口愈合的潜力尚未得到详细研究。因此，对于研究PKEO的生物活性的详细机理和分子方面可能具有吸引力。考虑到本研究是第一个报告科氏假单胞菌生物学活性的研究，需要对其代谢物进行进一步研究以复制本研究结果。