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A new alternative assay for sensitive analysis of ethylenethiourea and propylenethiourea in fruit samples after their separation.
Analytical Methods ( IF 3.1 ) Pub Date : 2020-07-07 , DOI: 10.1039/d0ay01001d
Siriwan Nantaphol 1 , Chochanon Moonla , Siriwipa Promvichai , Tanin Tangkuaram , Orawon Chailapakul , Weena Siangproh
Affiliation  

Ultra-high-performance liquid chromatography (UHPLC) coupled with a cobalt phthalocyanine screen-printed carbon electrode (CoPc-SPCE) was developed and validated for quantitative analysis of ethylenethiourea (ETU) and propylenethiourea (PTU). CoPC-SPCE provided high catalytic properties for ETU and PTU oxidation. This fabricated electrode is inexpensive, disposable, and easy to prepare by an in-house screen-printing technique. The chromatographic separation was performed in isocratic mode on a reversed phase C18 (100 mm × 4.6 mm, 3 μm) column, using a 90 : 10 (v/v) ratio of 0.05 M phosphate buffer solution (pH 4) and methanol as the mobile phase with a flow rate of 1.0 mL min−1 at an oxidation potential of +0.7 V vs. Ag/AgCl. The separation could be achieved within 3 min, and a wide linear range of 0.01–100 μg mL−1 (r2 > 0.99) was obtained for both analytes. The limits of detection (3 S/N) were found to be 0.006 and 0.009 μg mL−1 for ETU and PTU, respectively. Furthermore, this proposed method was utilized to determine ETU and PTU in fruit samples with satisfactory results, yielding excellent intra-day and inter-day relative standard deviations and recoveries. These results demonstrated that the proposed assay can be used as a new alternative way for inexpensive, rapid, selective and sensitive determination of ETU and PTU in fruit samples.

中文翻译:

分离后用于水果样品中亚乙基硫脲和亚丙基硫脲的灵敏分析的另一种替代测定方法。

开发了结合钴酞菁丝网印刷碳电极(CoPc-SPCE)的超高效液相色谱(UHPLC),并验证了该方法可用于亚乙基硫脲(ETU)和亚丙基硫脲(PTU)的定量分析。CoPC-SPCE为ETU和PTU氧化提供了高催化性能。这种制造的电极便宜,可抛弃,并且易于通过内部丝网印刷技术制备。色谱分离是在等度模式下在反相C18(100 mm×4.6 mm,3μm)色谱柱上进行的,色谱柱使用90:10(v / v)的0.05 M磷酸盐缓冲液(pH 4)和甲醇作为洗脱液。流动相,流速为1.0 mL min -1,氧化电位为+0.7 V,相对于Ag / AgCl。可以在3分钟内完成分离,并且两种分析物的线性范围都很广,为0.01–100μgmL -1r 2 > 0.99)。发现ETU和PTU的检出限(3 S / N)分别为0.006和0.009μgmL -1。此外,该方法被用于测定水果样品中的ETU和PTU,结果令人满意,可产生出色的日内和日间相对标准偏差和回收率。这些结果表明,所提出的测定方法可以用作廉价,快速,选择性和灵敏地测定水果样品中ETU和PTU的新替代方法。
更新日期:2020-07-30
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