Cleft palate (CP), a congenital defect in the oral and maxillofacial regions, is difficult to detect prenatally. This study investigated the correlation between differentially expressed proteins in serum and CP induced by all-trans retinoic acid (atRA) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in mice. We studied 80 mice in the following groups: male mice (male; n = 6), nonpregnant female control mice (NP-CRL; n = 6), healthy pregnant controls (P-CRL; Con; n = 24), pregnant mice with CP induced by atRA (n = 24), or pregnant mice with CP induced by TCDD (TCDD; n = 20). Pregnant mice were given with atRA (100 mg/kg) or TCDD (40 μg/kg), or corn oil by oral gavage at E10.5. The serum samples were collected and eight proteins—including interleukin (IL)-12p40, IL-12p70, receptor for advanced glycation end products (RAGE), interferon (IFN)-γ, IFN-β, IL-10, leukemia inhibitory factor (LIF), and epiregulin—were detected by enzyme-linked immunosorbent assay. Placental tissues were immunostained for IL-12p40 and RAGE from stages E13.5 to E16.5. In P-CRL mice, serum IL-12p40 was significantly increased at E13.5 and declined over E14.5–E16.5. P-CRL had lower IFN-γ levels at E13.5 compared with NP-CRL. The CP groups showed lower concentrations of IL-12p40 at E13.5–E14.5 and clearly higher concentrations of soluble RAGE (sRAGE) at E13.5 when compared with P-CRL. IL-12p40 immunostaining clearly decreased in placental tissue sections obtained from E13.5 to E14.5 in both CP groups. These findings suggest that reduced levels of IL-12p40 and increased levels of sRAGE in serum may be correlated with chemically induced CP in mice, but further studies would be required to establish this.

the裂（CP）是口腔和颌面部的先天性缺陷，很难在产前检测到。本实验研究在血清和CP差异表达的蛋白质由全反式维甲酸（ATRA）和2,3,7,8-四氯代诱导之间的相关性p小鼠二氧杂环己烯（TCDD）。我们研究了以下几组中的80只小鼠：雄性小鼠（雄性；n = 6），未怀孕的雌性对照小鼠（NP-CRL；n = 6），健康的怀孕对照（P-CRL； Con；n = 24），怀孕的小鼠由atRA诱导的CP（n = 24），或由TCDD诱导的CP的怀胎小鼠（TCDD; n= 20）。妊娠小鼠在E10.5处通过口管饲喂法给予atRA（100 mg / kg）或TCDD（40μg/ kg）或玉米油。收集了血清样品并检测了八种蛋白质，包括白介素（IL）-12p40，IL-12p70，晚期糖基化终末产物受体（RAGE），干扰素（IFN）-γ，IFN-β，IL-10，白血病抑制因子（ LIF）和上皮调节蛋白-通过酶联免疫吸附法检测。从阶段E13.5至E16.5对胎盘组织进行IL-12p40和RAGE免疫染色。在P-CRL小鼠中，血清IL-12p40在E13.5时明显升高，而在E14.5-E16.5时下降。与NP-CRL相比，P-CRL在E13.5的IFN-γ水平较低。与P-CRL相比，CP组在E13.5–E14.5处的IL-12p40浓度较低，而在E13.5处的可溶性RAGE（sRAGE）浓度较高。在两个CP组中，从E13.5至E14.5获得的胎盘组织切片中IL-12p40免疫染色明显降低。这些发现表明，小鼠血清中IL-12p40水平降低和sRAGE水平升高可能与化学诱导的小鼠CP相关，但是要确定这一点还需要进一步的研究。