Regulatory T cell (Treg) lineage plays a central role in inflammation and autoimmunity control. Interleukin-10 (IL-10) has been described as a pleiotropic cytokine that is mainly released by CD4⁺ CD25⁺ FOXP3⁺ Treg cells and has a potent immunosuppressive activity. Forkhead box P3 (FOXP3) transcription factor expression is crucial for Treg to function as a suppressor cell, and FOXP3 gene single nucleotide variants (SNVs) have already been shown to influence on viral pathogenesis. This study was conducted to evaluate the plasmatic and cervical levels of IL-10 in human papillomavirus-infected and uninfected patients and investigate whether the FOXP3 intron -1 SNVs rs3761548 and rs2232365 might alter IL-10 secretion. SNVs were genotyped by the characterization of polymerase chain reaction (PCR) products based on sequence-specific enzymatic cleavage using restriction fragment length polymorphism (RFLP) method. IL-10 levels were determined by quantitative enzyme-linked immunosorbent assay (ELISA). In conclusion, the data indicate that there is no association between FOXP3 SNVs and circulating and cervical IL-10 levels. This finding provides a rationale that IL-10 gene activation is independent of FOXP3 transcription factor activities on Treg cells.

中文翻译：

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FOXP3 遗传变异不影响女性人乳头瘤病毒感染的循环和宫颈白细胞介素 10 水平。

调节性 T 细胞 (Treg) 谱系在炎症和自身免疫控制中起着核心作用。Interleukin-10 (IL-10) 被描述为一种多效性细胞因子，主要由 CD4 ⁺ CD25 ⁺ FOXP3 ⁺ Treg 细胞释放，具有强大的免疫抑制活性。Forkhead box P3 (FOXP3) 转录因子表达对于 Treg 作为抑制细胞发挥作用至关重要，并且FOXP3基因单核苷酸变异 (SNV) 已被证明会影响病毒的发病机制。本研究旨在评估人乳头瘤病毒感染和未感染患者的血浆和宫颈 IL-10 水平，并调查FOXP3内含子 -1 SNV rs3761548 和 rs2232365 可能会改变 IL-10 的分泌。使用限制性片段长度多态性 (RFLP) 方法，通过基于序列特异性酶切的聚合酶链反应 (PCR) 产物的表征对 SNV 进行基因分型。IL-10 水平通过定量酶联免疫吸附试验 (ELISA) 确定。总之，数据表明FOXP3 SNV 与循环和宫颈 IL-10 水平之间没有关联。这一发现提供了一个基本原理，即IL-10基因激活独立于 Treg 细胞上的 FOXP3 转录因子活性。