ABSTRACT

Plants can be severely affected by heat stress due to rapid environmental changes. Use of endophytic fungi is a new tool to protect crops from the environmental stresses. Here we claim a potent endophyte isolated from Sonchus asper L. The aim was to explore the stress adaptive mechanism of sunflower and soybean mediated by Aspergillus niger (SonchL-7) under high temperature. Inoculation with A. niger boosted plant height, biomass and chlorophyl contents, while significantly curtailed the concentration of lipid peroxidation and reactive oxygen species (ROS) under thermal stress at 40°C. Moreover, the ROS-scavenging activities, like ascorbic acid oxidase (AAO), catalase (CAT), glutathione reductase (GR), superoxide dismutase (SOD) and peroxidase (POD) were augmented. Also, proline and phenolics were enhanced in the tested crops, while ABA concentration was significantly reduced. These positive results suggested that A. niger can be used as a heat-stress ameliorative tool for crops in the future.

摘要

由于快速的环境变化，植物可能会受到热胁迫的严重影响。使用内生真菌是一种保护农作物免受环境压力的新工具。在这里，我们要求保护一种有效的从Sonchus asper L分离的内生菌。目的是探索黑曲霉（SonchL-7）在高温下介导的向日葵和大豆的胁迫适应机制。黑曲霉接种增加了植物的高度，生物量和叶绿素含量，同时在40°C的热胁迫下显着降低了脂质过氧化和活性氧（ROS）的浓度。此外，增加了ROS清除活性，如抗坏血酸氧化酶（AAO），过氧化氢酶（CAT），谷胱甘肽还原酶（GR），超氧化物歧化酶（SOD）和过氧化物酶（POD）。同样，被测作物中脯氨酸和酚类物质含量增加，而ABA浓度则明显降低。这些积极的结果表明，黑曲霉可以在将来用作作物的热应激改善工具。