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Transcriptome signature of two lactation stages in Ghezel sheep identifies using RNA-Sequencing.
Animal Biotechnology ( IF 3.7 ) Pub Date : 2020-07-07 , DOI: 10.1080/10495398.2020.1784185
Mohammad Farhadian 1 , Seyed Aabbas Rafat 1 , Bahman Panahi 2 , Esmaeil Ebrahimie 3, 4
Affiliation  

The expression of genes and their regulation during lactation in Ghezel sheep breed remains less understood. To explore the underlying molecular mechanism of the lactation process in the mammary gland, transcriptome profiles of Iranian fat-tailed Ghezel sheep breed milk at two stages, before (BF) and after peak (AF) stages of lactation were investigated. Functional impacts of differentially expressed genes (DEGs) between BF and AF stages were surveyed using Gene Ontology (GO) and Protein-Protein Interaction (PPI) network analysis. Totally, 75 DEGs were identified between BF and AF stages of lactation. The RNA-Seq results were validated by Q-RT-PCR. Gene ontology of DEGs mainly enriched in metabolic process and oxidative phosphorylation. PPI network analysis also highlighted the contribution of peroxisome proliferator-activated receptors (PPAR) signaling, oxidative phosphorylation and metabolic pathways in the lactation process. Intriguingly, the genes involved in fat metabolism dominantly down-regulated at AF stage. Our results provide new insight into transcriptional changes and add to growing body of knowledge on the lactation process in fat-tailed sheep breeds.



中文翻译:

使用 RNA 测序鉴定 Ghezel 羊两个泌乳阶段的转录组特征。

Ghezel 绵羊品种在泌乳期间的基因表达及其调控仍然知之甚少。为了探索乳腺泌乳过程的潜在分子机制,研究了伊朗肥尾 Ghezel 羊在泌乳高峰期 (BF) 和高峰期 (AF) 两个阶段产奶的转录组谱。使用基因本体论 (GO) 和蛋白质-蛋白质相互作用 (PPI) 网络分析调查了 BF 和 AF 阶段之间差异表达基因 (DEG) 的功能影响。在哺乳期的 BF 和 AF 阶段之间总共确定了 75 个 DEG。RNA-Seq 结果通过 Q-RT-PCR 验证。DEGs的基因本体主要集中在代谢过程和氧化磷酸化。PPI 网络分析还强调了过氧化物酶体增殖物激活受体 (PPAR) 信号传导、氧化磷酸化和代谢途径在泌乳过程中的贡献。有趣的是,参与脂肪代谢的基因在 AF 阶段主要下调。我们的研究结果为转录变化提供了新的见解,并增加了关于肥尾羊泌乳过程的知识体系。

更新日期:2020-07-07
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