Abstract Context Salvia semiatrata Zucc. (Lamiaceae) is a species used as a tranquilizer and to relieve pain in folk medicine in Santiago Huauclilla, Oaxaca, Mexico. Objective To evaluate the antinociceptive and anxiolytic-like effects of S. semiatrata extracts and identify a bioactive metabolite. Materials and methods The extracts were obtained by maceration of S. semiatrata aerial parts using solvents in increasing polarity (hexane, ethyl acetate and methanol). A neo-clerodane diterpene was extracted from the ethyl acetate fraction using open column chromatography. Identification of this metabolite was performed by crystallography, 1H NMR, 13C NMR, ATR-IR, ECD, MS and elemental analysis. The antinociceptive activity was explored using the writhing and formalin tests. Whereas, the anxiolytic-like responses were analysed in the open-field, hole-board and plus-maze tests. All the treatments were administered using oral gavage in male CD1 mice and explored 30 min after administration of the individual extracts (300 mg/kg) or the compound 1 (0.1, 1 or 10 mg/kg). Results All the extracts produced significant reduction in the nociceptive and anxiety-like behaviour compared to mice treated with the vehicle (0.5% tween 80 in saline solution). The spectroscopic analysis corroborated the presence of the neo-clerodane diterpene 7-keto-neoclerodan-3,13-dien-18,19:15,16-diolide (1), as partial responsible of the antinociceptive and anxiolytic-like effects, which produced a dose-dependent response in the writhing test with an ED50=4.15 mg/kg. Discussion and conclusions: These results reinforce the medicinal properties of S. semiatrata in folk medicine, where participation of a neo-clerodane diterpene was evidenced in the inhibitory central nervous system activity of this species.

中文翻译：

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来自丹参地上部分的新克罗丹二萜的镇痛和抗焦虑样作用

摘要语境 Salvia semiatrata Zucc。（唇形科）是一种在墨西哥瓦哈卡州圣地亚哥华克利拉的民间医学中用作镇静剂和缓解疼痛的物种。目的评价半边莲提取物的镇痛和抗焦虑样作用，并鉴定一种生物活性代谢物。材料和方法 提取物是通过使用极性增加的溶剂（己烷、乙酸乙酯和甲醇）浸渍 S. semiatrata 地上部分获得的。使用开放式柱色谱法从乙酸乙酯级分中提取新克罗丹二萜。该代谢物的鉴定通过晶体学、1H NMR、13C NMR、ATR-IR、ECD、MS 和元素分析进行​​。使用扭体和福尔马林试验探索镇痛活性。而在开放场中分析抗焦虑样反应，孔板和加迷宫测试。所有治疗均在雄性 CD1 小鼠中使用口服管饲法给药，并在给药单个提取物 (300 mg/kg) 或化合物 1 (0.1、1 或 10 mg/kg) 后 30 分钟进行研究。结果与用赋形剂（0.5% 吐温 80 盐水溶液）处理的小鼠相比，所有提取物均显着降低了伤害性和焦虑样行为。光谱分析证实了新克罗丹二萜 7-keto-neoclerodan-3,13-dien-18,19:15,16-diolide (1) 的存在，作为镇痛和抗焦虑样作用的部分原因，在扭体试验中产生剂量依赖性反应，ED50 = 4.15 mg/kg。讨论和结论：这些结果加强了 S. semiatrata 在民间医学中的药用特性，