Soybean peroxidase is an enzyme extracted from soybean seed hulls. In the presence of hydrogen peroxide, the enzyme has the potential to catalyze the biodegradation of toxic substances like chlorophenols. For this reason, its use in wastewater treatment processes is environmentally friendly since the enzyme can be obtained from a renewable and abundant raw material. In this work, enzymatic biodegradation of 2,4,6-trichlorophenol performed by soybean peroxidase in a microreactor was studied experimentally and theoretically. The experimental data set was obtained with a volume of 250 μL by using different soybean peroxidase concentrations and different reaction times. The fluid dynamics of the microreactor was modeled as well, using ANSYS CFX. The simulations exhibited secondary flows, which enhanced mixing. Although the laminar flow was developed, it can be assumed to be a well-mixed medium. The kinetic data were evaluated through a mechanistic model, the modified bi-bi ping-pong model, which is adequate to represent the enzymatic degradation using peroxidases. The model was composed of an initial value problem for ordinary differential equations that were solved using MATLAB. Some kinetic constants were estimated using the least square function. The results of the model fit well the experimental data.

中文翻译：

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使用大豆过氧化物酶在微反应器中酶促降解 2,4,6-三氯苯酚

大豆过氧化物酶是一种从大豆种子壳中提取的酶。在过氧化氢存在的情况下，该酶有可能催化有毒物质（如氯酚）的生物降解。出于这个原因，它在废水处理过程中的使用是环保的，因为酶可以从可再生和丰富的原材料中获得。在这项工作中，通过实验和理论研究了大豆过氧化物酶在微反应器中酶促生物降解 2,4,6-三氯苯酚。通过使用不同的大豆过氧化物酶浓度和不同的反应时间，获得体积为 250 μL 的实验数据集。微反应器的流体动力学也使用 ANSYS CFX 进行建模。模拟表现出二次流动，这增强了混合。虽然层流发展起来，可以假设它是一种混合良好的介质。动力学数据通过机械模型进行评估，改进的 bi-bi ping-pong 模型足以代表使用过氧化物酶的酶促降解。该模型由使用 MATLAB 求解的常微分方程的初值问题组成。使用最小二乘函数估计一些动力学常数。模型结果与实验数据吻合较好。使用最小二乘函数估计一些动力学常数。模型结果与实验数据吻合较好。使用最小二乘函数估计一些动力学常数。模型结果与实验数据吻合较好。