Piscine orthoreovirus infects various salmonid fish species, and the infection is associated with diseases such as heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar). There are no vaccines available or genetically selected resistant hosts that can efficiently control piscine orthoreovirus (PRV) infection. Currently, the only prophylactic measure against PRV is general biosecurity measures aiming to break the transmission cycle. Methods to eradicate infectious virus from contaminated facilities are desirable, but the knowledge on how to inactivate PRV is lacking. A major bottleneck for inactivation studies is the lack of ability to propagate PRV in cell culture. Therefore, in this study we developed an in vivo model for detection of infectious PRV particles after treatment of the virus with inactivation tools such as heat, pH, iodine, UV and commercially available disinfectants. The results show that standard iodine treatment is efficient in inactivation of the virus, and similarly are high and low pH extremes and treatment with Virocid, a commercially available disinfectant. A UV dose of at least 50 mJ/cm² is required for inactivation, and the virus has high resistance against heat treatment.

中文翻译：

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灭活鱼正咽病毒。

鱼类正呼肠孤病毒感染各种鲑鱼，这种感染与养殖大西洋鲑鱼（Salmo salar）的疾病有关，例如心脏和骨骼肌炎症（HSMI））。没有可用的疫苗或遗传选择的抗性宿主，它们可以有效地控制鱼正咽病毒（PRV）感染。当前，针对PRV的唯一预防措施是旨在打破传播周期的一般生物安全措施。从受污染的设施中根除传染性病毒的方法是可取的，但是缺乏如何使PRV灭活的知识。灭活研究的主要瓶颈是缺乏在细胞培养物中繁殖PRV的能力。因此，在这项研究中，我们开发了一种体内模型，用于在用灭活工具（例如加热，pH，碘，紫外线和市售消毒剂）处理病毒后检测感染性PRV颗粒。结果表明，标准碘处理可有效灭活病毒，高和低pH极限以及使用市售消毒剂Virocid进行的处理类似。紫外线剂量至少为50 mJ / cm灭活需要^2个，并且病毒对热处理具有很高的抵抗力。