Cerium oxide-based nanozymes have recently drawn much attention in the field of biomedical and antioxidant applications, because of their unique regenerative or autocatalytic properties. Herein, we studied the biomimetic superoxide dismutase (SOD) nanozymes CeO₂-Gd that combines the fluorescence properties of rare earth Gd with the antioxidant properties of CeO₂ nanoparticles, which was prepared via facile route. The structure and composition of the CeO₂-Gd were measured and verified by X-ray powder diffraction (XRD), Raman spectroscopy, transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDX), and X-ray electron spectroscopy (XPS). Confocal microscopy was used to image cells. Antioxidant performance and cell viability of these nanozymes were measured in vitro using BGC-803 cell. CeO₂-Gd nanozymes with a higher Ce³⁺/Ce⁴⁺ ratio show higher superoxide dismutase (SOD) mimetic activity. Their antioxidant activity and fluorescence properties of CeO₂-Gd in BGC-803 cancer cells are enhanced by oxygen vacancies generated by doping rare-earth elements Gd. This work may guide the future design of CeO₂-Gd-based biomimetic nanozymes for anticancer and antioxidant applications.

由于其独特的再生或自催化特性，基于氧化铈的纳米酶最近在生物医学和抗氧化应用领域引起了广泛关注。在此，我们研究了仿生超氧化物歧化酶 (SOD) 纳米酶 CeO ₂ -Gd，它结合了稀土 Gd 的荧光特性和 CeO ₂纳米颗粒的抗氧化特性，通过简便的途径制备。CeO ₂的结构和组成-Gd 通过 X 射线粉末衍射 (XRD)、拉曼光谱、透射电子显微镜 (TEM)、能量色散 X 射线光谱 (EDX) 和 X 射线电子能谱 (XPS) 进行测量和验证。共聚焦显微镜用于对细胞成像。使用 BGC-803 细胞在体外测量这些纳米酶的抗氧化性能和细胞活力。具有较高Ce ³⁺ /Ce ⁴⁺比率的CeO ₂ -Gd 纳米酶表现出较高的超氧化物歧化酶(SOD) 模拟活性。CeO ₂ -Gd 在 BGC-803 癌细胞中的抗氧化活性和荧光特性通过掺杂稀土元素 Gd 产生的氧空位增强。这项工作可能会指导 CeO ₂的未来设计-用于抗癌和抗氧化应用的基于 Gd 的仿生纳米酶。