The DNA-binding with one finger (Dof) proteins are transcription factors involved in many biological processes in plants. To predict the evolutionary pattern, a genome-wide in-silico analysis of Dof TFs family in diploid (Gossypium arboreum and Gossypium raimondii) and allotetraploid (Gossypium hirsutum and Gossypium barbadense) cotton species were carried out. In G. arboreum, we have identified 58 non-redundant genes encoding Dof proteins renamed as GaDof (G. arboreum Dof), 55 Dof genes were identified in G. raimondii (GrDof), 89 were predicted ffrom G. hirsutum (GhDof) and the highest, 110 Dof genes were identified in G. barbadense (GbDof). The phylogenetic analysis, physical location, gene structure, conserved domain analyses were also investigated for G. arboreum, G. raimondii, and G. hirsutum. The gene expression pattern in G. hirsutum, at different growth stages, revealing the probable involvement of some GhDof genes in growth and development. These genes may improve seed germination and growth in cotton.

单指 DNA 结合 (Dof) 蛋白是参与植物许多生物过程的转录因子。为了预测进化模式，对二倍体（Gossypium arboreum和Gossypium raimondii）和异源四倍体（Gossypium hirsutum 和 Gossypium barbadense）棉花物种中的 Dof TFs 家族进行了全基因组计算机分析。在G. arboreum中，我们鉴定了58个编码Dof蛋白的非冗余基因，改名为GaDof ( G. arboreum Dof )，在G. raimondii ( GrDof )中鉴定出55个Dof基因， 89个来自G. hirsutum (GhDof ) 和最高的 110 个 Dof 基因在G. barbadense ( GbDof ) 中被鉴定。还研究了G. arboreum、G. raimondii和G. hirsutum的系统发育分析、物理定位、基因结构、保守域分析。G. hirsutum中不同生长阶段的基因表达模式，揭示了一些GhDof基因可能参与生长和发育。这些基因可能会改善棉花种子的发芽和生长。