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Deciphering the toxic effects of metals in gold mining area: Microbial community tolerance mechanism and change of antibiotic resistance genes.
Environmental Research ( IF 8.3 ) Pub Date : 2020-07-07 , DOI: 10.1016/j.envres.2020.109869
Changchun Yan 1 , Fei Wang 1 , Haijun Liu 2 , Huafeng Liu 3 , Shengyan Pu 4 , Fanyu Lin 5 , Huanhuan Geng 1 , Shuai Ma 1 , Yiyue Zhang 1 , Zhijun Tian 6 , Huilun Chen 1 , Beihai Zhou 1 , Rongfang Yuan 1
Affiliation  

Mine tailing dumps represent significant threats to ecological environments due to the presence of toxic substances. The present work investigated the relationship among microbial activity, the community, antibiotic resistance genes (ARGs) and trace metals in soil surrounding gold mine tailings. Using microbial metabolic activity and high-throughput sequencing analysis, we found the trace metals Cd and Hg could be main factors influencing the microbial community. According to bacterial co-occurrence pattern analysis, the effects of total cadmium and total mercury on bacterial diversity are potentially mediated by influencing bacteria community in the keystone module II. Additionally, most of metal-resistant bacteria belong to Actinobacteria and Proteobacteria, and the metal tolerance suggested to be linked with various functions including replication, recombination and repair, as well as inorganic ion transport and metabolism based on PICRUSt2 analysis. We also found that metals generated by mining activity may trigger the co-selection of antibiotic resistance in the phyla Actinobacteria and Proteobacteria due to co-resistance or cross resistance. Additionally, PLS-PM analysis revealed that metals could indirectly affect ARGs by influencing bacterial diversity in gold mining areas.



中文翻译:

解读金矿地区金属的毒性作用:微生物群落耐受机制和抗生素抗性基因的变化。

由于有毒物质的存在,矿山尾矿场对生态环境构成了重大威胁。本研究调查了金矿尾矿周围土壤中微生物活性,群落,抗生素抗性基因(ARGs)和微量金属之间的关系。通过微生物代谢活性和高通量测序分析,我们发现痕量金属镉和汞可能是影响微生物群落的主要因素。根据细菌共生模式分析,总镉和总汞对细菌多样性的影响可能是通过影响基石模块II中的细菌群落而介导的。此外,大多数对金属有抗性的细菌都属于放线菌和变形杆菌,根据PICRUSt2分析,金属耐受性与多种功能有关,包括复制,重组和修复以及无机离子转运和代谢。我们还发现,由于共耐药性或交叉耐药性,采矿活动产生的金属可能会触发门系放线菌和变形杆菌中抗生素抗性的共选。此外,PLS-PM分析表明,金属可能通过影响金矿区的细菌多样性而间接影响ARG。

更新日期:2020-07-14
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