The widespread detection of 17β-estradiol (E2) in the environment has become an emerging concern worldwide due to its endocrine disrupting effects. This work focuses on the aerobic and anaerobic biodegradations of E2 in various sedimentary environments with different availabilities of electron acceptors, including O₂, NO₃⁻, Fe³⁺, SO₄²⁻, or HCO₃⁻. The highest removal efficiency (98.9%) and shortest degradation half-life of E2 (t_1/2 = 5.0 d) were achieved under aerobic condition, followed by nitrate-reducing, ferric-reducing, sulfate-reducing and methanogenic conditions. We propose four different degradation pathways of E2 based on the metabolites identified under various redox conditions. Although most of E2 was effectively removed under aerobic condition, the potential environmental risk still needs to be considered due to the residual estrogenic activity induced by estrone (E1) formation. The endocrine-disrupting activities, as indicated by estradiol equivalent (EEQ) values, were related to E2 degradation rate and metabolite formation. We further analyzed the succession of bacterial community compositions and functions using Illumina HiSeq sequencing and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). The findings herein evidenced that bacterial community compositions and metabolic functions associated with different redox conditions impact the biodegradation of E2 and its endocrine-disrupting activity. This knowledge will be useful in predicting the environmental fates of estrogenic hormones in various sedimentary environments and aid in establishing appropriate strategies for eliminating potential environmental risks.

由于其内分泌干扰作用，在环境中对17β-雌二醇（E2）的广泛检测已成为全球关注的问题。此工作的重点是E2的与电子受体的不同可用性的各种沉积环境的需氧和厌氧biodegradations的，包括O- ₂，NO ₃ ^- ，铁³⁺，SO ₄ ^2-，或HCO ₃ ^- 。E2的最高去除效率（98.9％）和最短的降解半衰期（t _1/2 在有氧条件下达到= 5.0 d），然后进行硝酸盐还原，三价铁还原，硫酸盐还原和产甲烷条件。我们基于在各种氧化还原条件下鉴定出的代谢物提出了E2的四种不同降解途径。尽管大多数E2在有氧条件下被有效去除，但由于雌酮（E1）的形成会诱发残留的雌激素活性，因此仍需考虑潜在的环境风险。如雌二醇当量（EEQ）值所示，破坏内分泌的活动与E2降解率和代谢产物形成有关。我们进一步分析了细菌群落组成和功能的继承，使用Illumina HiSeq测序和通过未观察到的状态重建（PICRUSt）对群落进行系统发育研究。本文的发现证明与不同氧化还原条件有关的细菌群落组成和代谢功能影响E2的生物降解及其破坏内分泌的活性。该知识将有助于预测各种沉积环境中雌激素的环境命运，并有助于建立消除潜在环境风险的适当策略。