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The immune protection induced by a serine protease from the Trichinella spiralis adult administered as DNA and protein vaccine.
Acta Tropica ( IF 2.7 ) Pub Date : 2020-07-06 , DOI: 10.1016/j.actatropica.2020.105622
Daoxiu Xu 1 , Bin Tang 1 , Yang Wang 1 , Lixiao Zhang 1 , Zheng Qu 1 , Wenjie Shi 1 , Xuelin Wang 1 , Qingsong Sun 2 , Shumin Sun 3 , Mingyuan Liu 1
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Trichinellosis is caused by Trichinella spiralis (T. spiralis), which is an important public health problem. In this study, a gene encoding a serine protease from adult worms of T. spiralis (Ts-Adsp) was screened from a cDNA library of adult worms and was cloned and expressed in a prokaryotic expression system. The gene Ts-Adsp was subcloned into the eukaryotic expression vector pcDNA3.1(+), which was named pcDNA3.1(+)-Adsp. Previous studies have found that recombinant Ts-Adsp protein (rTs-Adsp) can elicit partial protection against T. spiralis infection in mice. Our aim was to explore the protective effect of combining a DNA vaccine with the rTs-Adsp protein against T. spiralis. One week after the last vaccination, the serum and spleen were obtained. The levels of IgG, IgG1 and IgG2a and cytokine production in serum and spleen cells were analyzed. The results showed that the levels of humoral and cell-mediated immune responses increased in the pcDNA3.1(+)-Adsp/rTs-Adsp group mice and demonstrated that a Th1/Th2 mixed immune response was induced by pcDNA3.1(+)-Adsp/rTs-Adsp after vaccination. Moreover, mice vaccinated with pcDNA3.1(+)-Adsp/rTs-Adsp displayed a 69.50% reduction in muscle larvae burden. This study suggested that mixed immunity could improve the muscle larvae reduction rate.



中文翻译:

成年旋毛虫的丝氨酸蛋白酶诱导的免疫保护作用,以DNA和蛋白质疫苗的形式给予。

旋毛虫病是由旋毛虫T. spiralis)引起的,旋毛虫是一个重要的公共卫生问题。在这项研究中,从成虫的cDNA文库中筛选出一种螺旋虫成虫的丝氨酸蛋白酶基因(Ts-Adsp),并将其克隆并在原核表达系统中表达。将Ts-Adsp基因亚克隆到真核表达载体pcDNA3.1(+)中,命名为pcDNA3.1(+)-Adsp。先前的研究发现重组Ts-Adsp蛋白(rTs-Adsp)可以引起部分抵抗小鼠螺旋体感染的保护作用。我们的目的是探索将DNA疫苗与rTs-Adsp蛋白结合起来对螺旋体的保护作用。在最后一次疫苗接种后一周,获得了血清和脾脏。分析血清和脾细胞中IgG,IgG1和IgG2a的水平以及细胞因子的产生。结果表明,pcDNA3.1(+)-Adsp / rTs-Adsp组小鼠的体液和细胞介导的免疫应答水平增加,并证明pcDNA3.1(+)诱导了Th1 / Th2混合免疫应答接种疫苗后-Adsp / rTs-Adsp。此外,接种了pcDNA3.1(+)-Adsp / rTs-Adsp的小鼠的肌肉幼虫负担减少了69.50%。这项研究表明,混合免疫可以提高肌肉幼虫的减少率。

更新日期:2020-07-10
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