Accurate mapping of transcription start sites (TSSs) is key for understanding transcriptional regulation. However, current protocols for genome-wide TSS profiling are laborious and/or expensive. We present Survey of TRanscription Initiation at Promoter Elements with high-throughput sequencing (STRIPE-seq), a simple, rapid, and cost-effective protocol for sequencing capped RNA 5′ ends from as little as 50 ng total RNA. Including depletion of uncapped RNA and reaction cleanups, a STRIPE-seq library can be constructed in about 5 h. We show application of STRIPE-seq to TSS profiling in yeast and human cells and show that it can also be effectively used for quantification of transcript levels and analysis of differential gene expression. In conjunction with our ready-to-use computational workflows, STRIPE-seq is a straightforward, efficient means by which to probe the landscape of transcriptional initiation.

中文翻译：

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使用STRIPE-seq进行转录起始和转录水平的简单有效分析。

转录起始位点（TSSs）的精确作图是理解转录调控的关键。但是，当前用于全基因组TSS分析的协议既费力又昂贵。我们提出了高通量测序（STRIPE-seq）在启动子元件上进行转录启动的调查，这是一种简单，快速且经济高效的方案，用于从低至50 ng的总RNA测序带帽RNA 5'末端。包括未封端RNA的消耗和反应清除，可以在大约5小时内构建STRIPE-seq文库。我们展示了STRIPE-seq在TSS分析中在酵母和人细胞中的应用，并表明它还可以有效地用于转录水平的定量和差异基因表达的分析。结合我们的即用型计算工作流程，STRIPE-seq十分简单，