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The fox tapeworm, Echinococcus multilocularis, in grey wolves and dogs in Slovakia: epidemiology and genetic analysis
Journal of Helminthology ( IF 1.6 ) Pub Date : 2020-07-06 , DOI: 10.1017/s0022149x20000528 J Jarošová 1, 2 , D Antolová 1 , V Šnábel 1 , N Guimarães 3 , J Štofík 4 , P Urban 5 , S Cavallero 6 , M Miterpáková 1
Echinococcus multilocularis , the causative agent of human alveolar echinococcosis, is an important emerging parasite in the northern hemisphere. In epidemiological studies, the highest attention is being paid to foxes as the main reservoir hosts responsible for geographic expansion from multiple focal populations and the invasion of urban habitats, but little information is available on the parasite distribution in other carnivores. Hence, the study was designed to obtain updated information about the occurrence and genetic diversity of E. multilocularis in grey wolves and dogs in Slovakia. Faecal samples of wolves were collected from three locations under a certain level of environmental protection in the central and eastern parts of the country, and the presence of the parasite DNA was detected in 35.7% of 112 samples, with the highest rate (51.2%) recorded in the Poloniny National Park in north-eastern Slovakia. Among 110 faecal dog samples, E. multilocularis was detected in three faeces from segregated Roma settlements in the eastern part of the country, which accounted for an overall positivity of 2.7%. Sequence analysis of two mitochondrial genes, 12S rRNA and NADH dehydrogenase subunit 1, revealed four haplotypes in 13 isolates from wolves and dogs originating from four sites in eastern and central Slovakia, with all samples bearing a European-type pattern of E. multilocularis . The more than one-third positivity rate of E. multilocularis in wolf faecal samples dispersed over a large part of the country has corroborated the extensive circulation of the parasite in wildlife and confirmed the need to improve intervention control strategies.
中文翻译:
斯洛伐克灰狼和狗体内的狐绦虫,多房棘球绦虫:流行病学和遗传分析
多房棘球绦虫 是人类肺泡包虫病的病原体,是北半球一种重要的新兴寄生虫。在流行病学研究中,最受关注的是狐狸作为主要宿主宿主,它们负责从多个焦点种群的地理扩张和城市栖息地的入侵,但关于其他食肉动物中寄生虫分布的信息很少。因此,该研究旨在获取有关该病的发生和遗传多样性的最新信息。E. multilocularis 斯洛伐克的灰狼和狗。狼粪便样本采集自中东部3个处于一定环境保护水平的地点,112份样本中检出率35.7%,检出率最高(51.2%)记录在斯洛伐克东北部的波洛尼尼国家公园。在 110 份粪便犬样本中,E. multilocularis 在该国东部被隔离的罗姆人定居点的三个粪便中检测到,总体阳性率为 2.7%。对两个线粒体基因 12S rRNA 和 NADH 脱氢酶亚基 1 的序列分析揭示了来自斯洛伐克东部和中部四个地点的狼和狗的 13 个分离株中的四个单倍型,所有样本都带有欧洲型模式E. multilocularis . 超过三分之一的阳性率E. multilocularis 分布在该国大部分地区的狼粪便样本证实了寄生虫在野生动物中的广泛传播,并证实需要改进干预控制策略。
更新日期:2020-07-06
Journal of Helminthology ( IF 1.6 ) Pub Date : 2020-07-06 , DOI: 10.1017/s0022149x20000528 J Jarošová 1, 2 , D Antolová 1 , V Šnábel 1 , N Guimarães 3 , J Štofík 4 , P Urban 5 , S Cavallero 6 , M Miterpáková 1
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中文翻译:
斯洛伐克灰狼和狗体内的狐绦虫,多房棘球绦虫:流行病学和遗传分析