ABSTRACT

As therapeutic antiviral agents, biological nanoparticles can fight the drug-resistant types of viruses helping the antiviral drug development. In this study, two blue-green algal strains; Oscillatoria sp. and Spirulina platensis were used, mediated by green Ag₂O|AgO-NPs and Au-NPs, respectively. For NPs characterization, the UV/Vis spectroscopy were used where their formation and crystallinity were proven with λ_max values for silver and gold NPs of 432 and 552 nm, respectively. The transmission electron microscope (TEM) X-ray diffraction showed a spherical-shaped Ag₂O|AgO-NPs (size; 14.42 to 48.97) while Au-NPs appeared with octahedral, pentagonal and triangular structures (size; 15.60–77.13 nm). The reducing, capping, and stabilization activities of algal polysaccharides and proteins were indicated via FTIR spectroscopy. Both Ag₂O|AgO-NPs and Au-NPs were investigated against Herpes Simplex virus (HSV-1) that has been indicated by its reduction activity of cytopathic effect (CPE). Cytotoxicity was evaluated on Vero cells and measured by MTT assay. Results showed a 90% reduction in CPE of HSV-1 applying Ag₂O|AgO-NPs, and Au-NPs at 31.25 μL., with a high reduction rate (49.23%) with Ag₂O|AgO-NPs than that of Au-NPs (42.75%). Current results proved the efficiency of green nanotechnology application with both Ag₂O|AgO-NPs, and Au-NPs as reducing and inhibitory agents for the HSV-1 replication.

摘要

作为治疗性抗病毒药物，生物纳米粒子可以对抗耐药病毒类型，有助于抗病毒药物的开发。在这项研究中，两种蓝绿藻菌株；震颤菌 使用了螺旋藻和螺旋藻，分别由绿色 Ag ₂ O|AgO-NPs 和 Au-NPs 介导。对于 NPs 表征，使用 UV/Vis 光谱，其中银和金 NPs 的λ_最大值分别为 432 和 552 nm，证明了它们的形成和结晶度。透射电子显微镜 (TEM) X 射线衍射显示球形 Ag ₂O|AgO-NPs（尺寸；14.42 至 48.97），而 Au-NPs 出现八面体、五边形和三角形结构（尺寸；15.60-77.13 nm）。通过 FTIR 光谱表明藻类多糖和蛋白质的还原、加帽和稳定活性。Ag ₂ O|AgO-NPs 和 Au-NPs 均针对单纯疱疹病毒 (HSV-1) 进行了研究，这已通过其减少细胞病变效应 (CPE) 的活性来证明。在 Vero 细胞上评估细胞毒性并通过 MTT 测定法测量。_{结果表明，使用 Ag 2} O|AgO-NPs 和 Au-NPs 31.25 μL，HSV-1 的 CPE 降低了 90%，其中 Ag ₂ O|AgO-NPs 的降低率（49.23%）高于Au-NPs (42.75%)。目前的结果证明了绿色纳米技术应用的效率₂ O|AgO-NPs 和 Au-NPs 作为 HSV-1 复制的还原剂和抑制剂。