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Insecticidal and insect growth regulatory activities of secondary metabolites from entomopathogenic fungi, Lecanicillium attenuatum
Journal of Applied Entomology ( IF 1.9 ) Pub Date : 2020-07-06 , DOI: 10.1111/jen.12788
Ra Mi Woo 1 , Min Gu Park 1 , Jae Young Choi 1 , Dong Hwan Park 1 , Jun Young Kim 1 , Minghui Wang 1 , Hyun Ji Kim 1 , Soo Dong Woo 2 , Jae Su Kim 3 , Yeon Ho Je 1, 4
Affiliation  

Insect growth regulators (IGRs) are effective alternatives to chemical insecticides because of their specificity and low environmental toxicity. Entomopathogenic fungi are an important natural pathogen of insects and have been developed as biological control agents. They produce a wide range of secondary metabolites such as antibiotics, pesticides, growth‐promoting or inhibiting compounds and insect attracting agents. In this study, to explore novel IGR substances from entomopathogenic fungi, culture extracts of 189 entomopathogenic fungi isolated from Korean soil samples were investigated for their juvenile hormone (JH)‐based IGR activities. Whereas none of the culture extracts exhibited JH agonist (JHA) activity, 14 extracts showed high levels of JH antagonist (JHAN) activity. Among them, culture extract of JEF‐145 strain, which was identified as Lecanicillium attenuatum, showed the highest insecticidal against Aedes albopictus and Plutella xylostella. At liquid culture condition, JHAN activity was observed in culture soup rather than mycelial cake, indicating that substances with JHAN activity are released from the JEF‐145 strain during culture. Furthermore, while extract from solid cultured JEF‐145 strain showed insecticidal activities against both A. albopictus and P. xylostella, that from liquid cultured fungi showed insecticidal activity only against A. albopictus, indicating that L. attenuatum JEF‐145 strain produces different kinds of secondary metabolites with JHAN activity depending on culture conditions. These results suggested that JHAN substances derived from entomopathogenic fungi could be usefully exploited to develop novel eco‐friendly IGR insecticides.

中文翻译:

昆虫致病性真菌Lecanicillium lutum的次生代谢产物的杀虫和昆虫生长调控活性

昆虫生长调节剂(IGR)是化学杀虫剂的有效替代品,因为它们具有特异性和低环境毒性。昆虫病原真菌是昆虫的重要自然病原体,已被开发为生物防治剂。它们产生各种次级代谢产物,例如抗生素,农药,促进生长或抑制的化合物以及引诱昆虫的物质。在这项研究中,为了探索来自昆虫病原真菌的新型IGR物质,对从韩国土壤样品中分离的189昆虫病原真菌的培养物提取物进行了基于少年激素(JH)的IGR活性研究。尽管所有培养物提取物均未显示出JH激动剂(JHA)活性,但14种提取物均显示出高水平的JH拮抗剂(JHAN)活性。其中包括JEF‐145菌株的培养物提取物,弱毒杆菌对白纹伊蚊小菜蛾有最高的杀虫作用。在液体培养条件下,在培养汤中观察到JHAN活性,而不是菌丝饼,这表明在培养过程中具有JEHAN活性的物质从JEF-145菌株中释放出来。此外,固相培养的JEF-145菌株的提取物对白曲霉小菜蛾均有杀虫活性,而液体培养的真菌的提取物仅对白菜曲霉有杀虫活性,表明减毒曲霉JEF‐145菌株会根据培养条件产生具有JHAN活性的不同种类的次级代谢产物。这些结果表明,来源于昆虫病原性真菌的JHAN物质可以有效地用于开发新型的生态友好型IGR杀虫剂。
更新日期:2020-07-06
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