Mycobacterium tuberculosis possesses the ability to undergo physiological adaptations in order to persist during the prolonged course of infection despite the active immune response of the host and in order to overcome multiple environmental changes. Previous studies have proposed that M. tuberculosis CuvA (Rv1422; MtCuvA) might play a critical role in the adaptation of the bacterium to environmental changes, such as nutrient utilization and alteration of the growth rate. However, the detailed function of MtCuvA still remains unclear owing to a lack of structural information. To better understand its role in host adaptation, MtCuvA was purified to homogeneity and was crystallized for the first time using the hanging‐drop vapor‐diffusion method. The crystal of MtCuvA diffracted to a resolution of 2.1 Å and belonged to the orthorhombic space group P2₁2₁2₁, with unit‐cell parameters a = 47.27, b = 170.93, c = 178.10 Å. The calculated Matthews coefficient (V_M) was 2.4 ų Da⁻¹, with a solvent content of 48.02%, and thus four molecules appeared to be present in the asymmetric unit. Moreover, it is reported that MtCuvA can bind to the cell‐wall precursor components uridine diphosphate (UDP)‐glucose and UDP‐N‐acetylglucosamine.

中文翻译：

---

一种来自结核分枝杆菌 H37Rv 的碳利用和毒力蛋白 A CuvA (Rv1422)：结晶、X 射线衍射分析和配体结合。

结核分枝杆菌具有进行生理适应的能力，以便在长期感染过程中持续存在，尽管宿主有积极的免疫反应，并克服多种环境变化。先前的研究提出，结核分枝杆菌CuvA（Rv1422；MtCuvA）可能在细菌适应环境变化（例如养分利用和生长速度的改变）中发挥关键作用。然而，由于缺乏结构信息，MtCuvA 的详细功能仍不清楚。为了更好地了解其在宿主适应中的作用，MtCuvA 被纯化至均质，并首次使用悬滴蒸气扩散法进行结晶。MtCuvA晶体的衍射分辨率为2.1 Å，属于斜方空间群P 2 ₁ 2 ₁ 2 ₁，晶胞参数a = 47.27，b = 170.93，c = 178.10 Å。计算出的马修斯系数( _VM )为2.4 Å ³  Da ^-1，溶剂含量为48.02%，因此不对称单元中似乎存在四个分子。此外，据报道，MtCuvA 可以与细胞壁前体成分尿苷二磷酸 (UDP)-葡萄糖和 UDP- N-乙酰氨基葡萄糖结合。