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Description, occurrence and significance of Mycoplasma seminis sp. nov. isolated from semen of a gyrfalcon (Falco rusticolus).
Veterinary Microbiology ( IF 3.3 ) Pub Date : 2020-07-06 , DOI: 10.1016/j.vetmic.2020.108789
Luisa Fischer 1 , Franca Möller Palau-Ribes 1 , Dirk Enderlein 1 , Dominik Fischer 1 , Werner Herbst 2 , Liane Baudler 1 , Hafez Mohamed Hafez 3 , Michael Lierz 1
Affiliation  

The Mycoplasma strain ARNO was isolated from the semen of a clinically healthy gyrfalcon (Falco rusticolus). Colonies of strain ARNO grew in fried-egg shape on Mycoplasma agar plates (SP4). The organism did not ferment glucose or hydrolyze arginine or urea; hence, organic acids are assumed as energy source. Growth was sterol-dependent and optimal growth temperature 42 °C, with a temperature range from 20 to 44 °C. Strain ARNO was not identified as a representative of any of the currently described Mycoplasma species by alignment of the 16S rRNA gene sequence and 16 S–23 S intergenic transcribed spacer region, or immunobinding assay. Hence, strain ARNO represents a novel Mycoplasma species for which the name Mycoplasma seminis sp. nov. is proposed (DSM 27653, NCTC 13927). After developing a species-specific PCR, the prevalence of M. seminis sp. nov. was determined in adult and juvenile falcons in a commercial breeding center for falcons. Semen samples (n = 171) were obtained from 113 male adults, due to repeated sampling of 39 birds. Female adults (n = 26) were sampled once, while 105 of the 152 juvenile birds were sampled twice via choanal swabs. Mycoplasma seminis sp. nov. was found in the semen of clinically healthy adult males (3.5 %) as well as in the respiratory tract of female (34.6 %) and juvenile birds (59.2 %). After comparison of semen samples with (2.9 %) and without M. seminis sp. nov. identification, no indications for a potential influence on the semen quality were demonstrated. Hence, M. seminis sp. nov. seems likely to be of commensal character in falcons.



中文翻译:

说明,发生和意义的支原体。十一月 从gyrfalcon(Falcorusticalus)的精液中分离。

支原体菌株ARNO是从临床健康隼(精液中分离的Falco rusticolus)。ARNO菌株的菌落在支原体琼脂平板(SP4)上呈煎蛋状生长。该生物没有发酵葡萄糖或水解精氨酸或尿素;因此,假定有机酸为能源。生长是固醇依赖性的,最佳生长温度为42°C,温度范围为20至44°C。通过比对16S rRNA基因序列和16 S-23 S基因间转录的间隔区,或进行免疫结合分析,ARNO菌株未被鉴定为任何目前描述的支原体种类的代表。因此,ARNO菌株代表一种新型支原体支原体圣尼斯SP。十一月 (DSM 27653,NCTC 13927)。在开发了物种特异性PCR后,M。seminis sp。的流行。十一月 是在商业猎鹰繁殖中心对成年和幼年猎鹰进行测定的。精液样本(n = 171)取自113羽成年雄性,因为要对39只鸡进行重复采样。成年雌性(n = 26)采样了一次,而152只幼鸟中的105羽是通过咽拭子采样了两次。支原体圣尼斯SP。十一月 在临床上健康的成年雄性精液(3.5%)以及雌性(34.6%)和幼鸟(59.2%)的呼吸道中发现了这种病毒。与(2.9%)精液样品的比较之后并且没有M.精囊sp。十一月 鉴定,没有迹象表明对精液质量有潜在影响。因此,M。seminis sp.。十一月 似乎可能是猎鹰的纪念人物。

更新日期:2020-07-08
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