The effectiveness of stem cell-based periodontal tissue engineering need to be assessed by preclinical animal studies. Dog models are widely used animal models; however, there are not sufficient data on characterization of canine dental mesenchymal stem cells. Therefore, we aimed to compare the characteristics among canine and human periodontal ligament stem cells and canine and human dental pulp stem cells. Canine periodontal ligament stem cells and dental pulp stem cells showed significantly weaker clonogenic capability, and proliferation and migration capacity, and they displayed lower positive rates for CD90, CD73, CD105, and STRO-1. All of these canine and human cells showed multilineage differentiation potential. After osteogenic induction, the expression of alkaline phosphatase was obviously upregulated in human dental mesenchymal stem cells, but it was not upregulated in canine dental pulp stem cells. Other osteogenic genes, such as runt-related transcription factor 2 and bone morphogenetic protein 2, were upregulated in all induced canine and human cells, but their upregulation occurred later in canine cells. These results confirmed the stem cell properties of canine mesenchymal stem cells, but also suggested that more attention should be paid to the choice of appropriate research approaches, osteogenic gene markers, and time points for the utilization of canine dental mesenchymal stem cells due to their distinct characteristics.

基于干细胞的牙周组织工程的有效性需要通过临床前动物研究进行评估。狗模型是广泛使用的动物模型；然而，关于犬牙间充质干细胞表征的数据不足。因此，我们旨在比较犬和人牙周膜干细胞与犬和人牙髓干细胞的特性。犬牙周膜干细胞和牙髓干细胞的克隆形成能力、增殖和迁移能力明显较弱，CD90、CD73、CD105和STRO-1的阳性率较低。所有这些犬和人类细胞都显示出多向分化潜能。成骨诱导后，碱性磷酸酶的表达在人牙间充质干细胞中明显上调，但在犬牙髓干细胞中未上调。其他成骨基因，如 runt 相关转录因子 2 和骨形态发生蛋白 2，在所有诱导的犬和人类细胞中上调，但它们在犬细胞中的上调发生较晚。这些结果证实了犬间充质干细胞的干细胞特性，但也表明，由于犬牙间充质干细胞的独特性，应更多地关注选择合适的研究方法、成骨基因标记和利用犬牙间充质干细胞的时间点。特征。例如矮小相关转录因子 2 和骨形态发生蛋白 2，在所有诱导的犬和人类细胞中均被上调，但它们在犬细胞中的上调发生较晚。这些结果证实了犬间充质干细胞的干细胞特性，但也表明，由于犬牙间充质干细胞的独特性，应更多地关注选择合适的研究方法、成骨基因标记和利用犬牙间充质干细胞的时间点。特征。例如矮小相关转录因子 2 和骨形态发生蛋白 2，在所有诱导的犬和人类细胞中均被上调，但它们在犬细胞中的上调发生较晚。这些结果证实了犬间充质干细胞的干细胞特性，但也表明，由于犬牙间充质干细胞的独特性，应更多地关注选择合适的研究方法、成骨基因标记和利用犬牙间充质干细胞的时间点。特征。