Silicon (Si) and selenium (Se), two beneficial elements that alleviate cadmium (Cd) toxicity, are important for agricultural production and human health. However, the effects and related mechanisms of Si-Se interaction on Cd toxicity alleviation are still poorly understood. Herein, a hydroponic experiment was employed to evaluate the effects of Si and Se alone and together, on the growth, Cd content, and biochemical parameters of Cd-treated rice plants. The results revealed that both Si and Se can effectively alleviate Cd toxicity, and a strong synergistic effect of Si and Se was observed. Simultaneous use of Si and Se significantly promoted rice plant growth, decreased malondialdehyde (MDA) content in both the roots and shoots, and reduced Cd translocation factor leading to a significant 73.2% decrease in shoot Cd content. Additionally, Si-Se interaction increased glutathione (GSH) content, phytochelatin (PC) content and Cd distribution in root cell walls and organelles. Furthermore, the relative expression of OsHMA2 was down-regulated, while those of OsNramp1 and OsMHA3 were up-regulated. The above findings suggest that synergistic effect of Si and Se on Cd toxicity amelioration occurs mainly via regulating gene expression, sequestering Cd in the root cell walls and organelles, and reducing Cd transfer to the shoots.

硅（Si）和硒（Se）是缓解镉（Cd）毒性的两个有益元素，对农业生产和人类健康至关重要。然而，Si-Se相互作用对减轻Cd毒性的影响及其相关机制仍知之甚少。在本文中，通过水培试验评估了单独和联合使用Si和Se对经Cd处理的水稻植株的生长，Cd含量和生化参数的影响。结果表明，Si和Se都可以有效减轻Cd的毒性，并且观察到Si和Se具有很强的协同作用。同时使用Si和Se可以显着促进水稻植物的生长，降低根和茎中的丙二醛（MDA）含量，并降低Cd的转运因子，从而导致茎中Cd含量显着降低73.2％。另外，Si-Se相互作用增加了根细胞壁和细胞器中的谷胱甘肽（GSH）含量，植物螯合素（PC）含量和Cd分布。此外，...的相对表达OsHMA2下调，而OsNramp1和OsMHA3上调。上述发现表明，Si和Se对Cd毒性改善的协同作用主要通过调节基因表达，将Cd螯合在根细胞壁和细胞器中以及减少Cd向芽的转移而发生。