Salvia viridis transformed root culture was studied using MS, WP or B5 media (full or half-strength macro- and microelements: 1/2MS, 1/2WP, 1/2B5) under varying light conditions to optimize growth and polyphenolic compound production. The highest levels of root growth (154.6 g/l and 13.6 g/l, respectively, fresh and dry weight) were observed for hairy roots grown in WP medium in the dark. UPLC analysis found the polyphenolic acid content in plant material to range from 20.7 to 45.9 mg/g dry weight (DW) depending on the conditions. The highest metabolite content was observed in roots grown in B5 medium under photoperiods. A time-course study of the hairy roots cultured in WP medium in the dark examined the effect of culture period on biomass and polyphenolic compound yield every 5 days over a 60-day period. More than 1 g of dry weight per flask and 47.8 mg of metabolite per g DW were produced after 30 days, at the beginning of the stationary phase. However, maximum production was only observed for four of the 10 detected compounds during this growth phase, between days 30 and 40; one of which was rosmarinic acid: the predominant compound in all extracts. Under optimal conditions, production was almost 10 times higher than that observed in the roots of field growing plants, which suggests that the selected optimized transformed root culture of S. viridis may be a good approach for valuable polyphenol production.

丹参使用MS，WP或B5培养基（完整或半强度的宏观和微量元素：1 / 2MS，1 / 2WP，1 / 2B5）在变化的光照条件下研究了转化的根培养物，以优化生长和多酚化合物的生产。在黑暗中，WP培养基中生长的毛状根的最高根生长水平（分别为新鲜和干重154.6 g / l和13.6 g / l）。UPLC分析发现，取决于条件，植物材料中的多酚酸含量范围为20.7至45.9 mg / g干重（DW）。在光周期下，在B5培养基中生长的根中观察到最高的代谢物含量。一项在黑暗中在WP培养基中培养的毛状根的时程研究研究了培养期对60天期内每5天对生物量和多酚化合物产量的影响。每个烧瓶和47瓶的干重超过1克。在静止期开始30天后，每克DW产生8毫克代谢产物。但是，在此生长阶段（第30天到40天之间），仅观察到10种检测到的化合物中的4种的最大产量；其中之一是迷迭香酸：所有提取物中的主要化合物。在最佳条件下，产量几乎是田间种植植物根部观察到的产量的10倍，这表明选择的优化转化根系培养物绿色葡萄球菌可能是有价值的多酚生产的好方法。