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Fluorescent AgInS/ZnS quantum dots microplate and lateral flow immunoassays for folic acid determination in juice samples
Microchimica Acta ( IF 5.7 ) Pub Date : 2020-07-06 , DOI: 10.1007/s00604-020-04398-1
Anastasiya S Novikova 1 , Тatiana S Ponomaryova 1 , Irina Yu Goryacheva 1
Affiliation  

A noninstrumental rapid test for folic acid (FA) detection with visual results evaluation utilizing bright water-stable AgInS/ZnS (AIS/ZnS) quantum dots (QDs) is reported . AIS/ZnS QDs are hydrophilic photostable nanocrystals with size < 7 nm and emission in the visible range. They were synthesized directly in the water phase by a simple method compared to the synthesis of other QDs and conjugated with monoclonal antibodies specific for FA via ligand carboxyl groups. The conjugate was used for the development of instrumental qualitative and rapid quantitative FA detection methods. The competitive fluorescent microplate immunosorbent assay provided a limit of detection of 0.1 ng/mL FA and half maximal inhibitory concentration (IC50) of 24 ng/mL FA. The analytical signal was measured at ʎex = 410 nm and ʎem=590 nm. The proposed method showed no cross-reaction with other group B vitamins. For FA screening in juice samples, the lateral flow immunoassay was developed with a visual cutoff level of 3 μg/mL. In our perception, the developed methods are convenient for proving the perception of the AIS/ZnS QDs application as a luminescent label for immunoassay and are effective for FA detection. Graphical abstract Graphical abstract

中文翻译:

用于果汁样品中叶酸测定的荧光 AgInS/ZnS 量子点微孔板和侧流免疫分析

叶酸 (FA) 检测的非仪器快速测试和视觉结果评估利用明亮的水稳定 AgInS/ZnS (AIS/ZnS) 量子点 (QDs) 报告。AIS/ZnS QD 是亲水性光稳定纳米晶体,尺寸 < 7 nm,在可见光范围内发射。与其他 QD 的合成相比,它们通过一种简单的方法直接在水相中合成,并通过配体羧基与对 FA 具有特异性的单克隆抗体偶联。该偶联物用于开发仪器定性和快速定量 FA 检测方法。竞争性荧光微孔板免疫吸附测定提供了 0.1 ng/mL FA 的检测限和 24 ng/mL FA 的半数最大抑制浓度 (IC50)。在 ʎex = 410 nm 和 ʎem = 590 nm 处测量分析信号。所提出的方法与其他 B 族维生素没有交叉反应。对于果汁样品中的 FA 筛选,开发了横向流动免疫测定法,其视觉截止水平为 3 μg/mL。在我们看来,所开发的方法便于证明 AIS/ZnS QD 应用作为免疫测定的发光标签的感知,并且对 FA 检测有效。图形摘要图形摘要
更新日期:2020-07-06
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