The allophycocyanin (APC) protein purified from Phormidium sp. A09DM was investigated for its in vivo antioxidant and anti-aging potential in Caenorhabditis elegans. An increased mean lifespan of APC-treated (100 μg/ml) worms (wild type) were observed from 16 ± 0.2 days (control) to 20 ± 0.1 days (treated). APC-treated worms also showed improved physiological marker of aging such as the rate of pharyngeal pumping and higher rate of survival against oxidative and thermal stress. Furthermore, APC was found to moderate the expression of human amyloid beta (Aβ1–42) as well as associated Aβ-induced paralysis in the transgenic C. elegans CL4176 upon increase in temperature. Furthermore, RNA interference (RNAi)-mediated studies revealed the dependence of downstream regulator daf-16, independent of stress-induced resistance gene skn-1 in the APC treated C. elegans. In the present study, we tried to demonstrate the anti-aging activity, longevity and protective effects of APC against cellular stress in C. elegans, which can lead to the use of this biomolecule in drug development for age-related disorders.

从Phormidium sp. 中纯化的别藻蓝蛋白 (APC) 蛋白。研究了 A09DM 在秀丽隐杆线虫中的体内抗氧化和抗衰老潜力。观察到 APC 处理 (100 μg/ml) 蠕虫（野生型）的平均寿命从 16 ± 0.2 天（对照）增加到 20 ± 0.1 天（处理）。经 APC 处理的蠕虫还显示出改善的衰老生理标志物，例如咽部泵血率和更高的抗氧化和热应激存活率。此外，发现 APC 可调节转基因秀丽隐杆线虫中人淀粉样蛋白 (Aβ1-42) 的表达以及相关的 Aβ 诱导的麻痹CL4176 在温度升高时。此外，RNA 干扰 (RNAi) 介导的研究揭示了下游调节器daf - 16的依赖性，与 APC 处理的秀丽隐杆线虫中的应激诱导抗性基因skn-1无关。在本研究中，我们试图证明 APC 对秀丽隐杆线虫细胞应激的抗衰老活性、长寿和保护作用，这可能导致该生物分子用于治疗年龄相关疾病的药物开发。