Premature ovarian failure (POF) is one of the principal causes of female infertility, and although its causes are complex and diverse, autoimmune deficiency may be involved. Human umbilical cord mesenchymal stem cells (UCMSCs) can be used for tissue regeneration and repair. Therefore, the present study was designed to determine the role of UCMSCs in immune factor-induced POF in rats. In this study, different concentrations of UCMSCs were injected into induced POF rats. Ovarian functions were examined by evaluating the estrus cycle, follicular morphology, hormonal secretion, and the proliferation and apoptosis of granulosa cells. Our results showed that the estrus cycle of rats returned to normal and follicular development was significantly improved after transplantation of UCMSCs. In addition, serum concentrations of 17-estradiol (E2), progesterone (P4), and anti-Müllerian hormone (AMH) increased significantly with treatment. Transplantation of UCMSCs also reduced the apoptosis of granulosa cells and promoted the proliferation of granulosa cells. All of these improvements were dose dependent. Furthermore, the results of related gene expression showed that transplanted human UCMSCs upregulated the expression of Bcl-2, AMH, and FSHR in the ovary of POF rats and downregulated the expression of caspase-3. These results further validated the potential mechanisms of promoting the release of cell growth factors and enhancing tissue regeneration and provide a theoretical basis for the clinical application of stem cells in the treatment of premature ovarian failure.

中文翻译：

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在人类免疫性卵巢早衰大鼠模型中，使用人脐带间充质干细胞疗法。

卵巢早衰（POF）是女性不育的主要原因之一，尽管其原因复杂多样，但可能涉及自身免疫缺陷。人脐带间充质干细胞（UCMSC）可用于组织再生和修复。因此，本研究旨在确定UCMSCs在免疫因子诱导的大鼠POF中的作用。在这项研究中，将不同浓度的UCMSCs注射入诱导的POF大鼠中。通过评估发情周期，卵泡形态，荷尔蒙分泌以及颗粒细胞的增殖和凋亡来检查卵巢功能。我们的结果表明，UCMSCs移植后大鼠的发情周期恢复正常，卵泡发育得到明显改善。此外，血清中的17-雌二醇（E2）黄体酮（P4）和抗苗勒氏激素（AMH）在治疗后显着增加。UCMSCs的移植也减少了颗粒细胞的凋亡并促进了颗粒细胞的增殖。所有这些改善都是剂量依赖性的。此外，相关基因表达的结果表明，移植的人UCMSCs上调了POF大鼠卵巢中Bcl-2，AMH和FSHR的表达，并下调了caspase-3的表达。这些结果进一步验证了促进细胞生长因子释放和增强组织再生的潜在机制，并为干细胞在卵巢早衰治疗中的临床应用提供了理论基础。UCMSCs的移植也减少了颗粒细胞的凋亡并促进了颗粒细胞的增殖。所有这些改善都是剂量依赖性的。此外，相关基因表达的结果表明，移植的人UCMSCs上调了POF大鼠卵巢中Bcl-2，AMH和FSHR的表达，并下调了caspase-3的表达。这些结果进一步验证了促进细胞生长因子释放和增强组织再生的潜在机制，并为干细胞在卵巢早衰治疗中的临床应用提供了理论基础。UCMSCs的移植也减少了颗粒细胞的凋亡并促进了颗粒细胞的增殖。所有这些改善都是剂量依赖性的。此外，相关基因表达的结果表明，移植的人UCMSCs上调了POF大鼠卵巢中Bcl-2，AMH和FSHR的表达，并下调了caspase-3的表达。这些结果进一步验证了促进细胞生长因子释放和增强组织再生的潜在机制，并为干细胞在卵巢早衰治疗中的临床应用提供了理论基础。相关基因表达结果表明，移植的人UCMSCs可上调POF大鼠卵巢中Bcl-2，AMH和FSHR的表达，并下调caspase-3的表达。这些结果进一步验证了促进细胞生长因子释放和增强组织再生的潜在机制，并为干细胞在卵巢早衰治疗中的临床应用提供了理论基础。相关基因表达结果表明，移植的人UCMSCs可上调POF大鼠卵巢中Bcl-2，AMH和FSHR的表达，并下调caspase-3的表达。这些结果进一步验证了促进细胞生长因子释放和增强组织再生的潜在机制，并为干细胞在卵巢早衰治疗中的临床应用提供了理论基础。